A simple, efficient method for the separation of humic substances and DNA from environmental samples

被引：137

作者：

Jackson, CR

Harper, JP

Willoughby, D

Roden, EE

Churchill, PF

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 12期

关键词：

D O I：

10.1128/AEM.63.12.4993-4995.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified by the other materials.

引用

页码：4993 / 4995

页数：3

共 22 条

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