Tensile forces govern germ-layer organization in zebrafish

被引:592
作者
Krieg, M. [1 ]
Arboleda-Estudillo, Y. [1 ,2 ]
Puech, P. -H. [3 ]
Kaefer, J. [4 ,5 ]
Graner, F. [4 ,5 ]
Mueller, D. J. [1 ]
Heisenberg, C. -P. [2 ]
机构
[1] Tech Univ Dresden, BIOTEC, D-01307 Dresden, Germany
[2] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
[3] INSERM, UMR 600, CNRS UMR6212, Case 937, F-13288 Marseille, France
[4] UJF Grenoble 1, UMR 5588, Spectrometrie Phys Lab, F-38402 St Martin Dheres, France
[5] Ecole Natl Super Electrochim & Electrome Grenoble, Thermodynam & Physicochim Met Lab, CNRS, F-38402 St Martin Dheres, France
关键词
D O I
10.1038/ncb1705
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Understanding the factors that direct tissue organization during development is one of the most fundamental goals in developmental biology. Various hypotheses explain cell sorting and tissue organization on the basis of the adhesive and mechanical properties of the constituent cells(1). However, validating these hypotheses has been difficult due to the lack of appropriate tools to measure these parameters. Here we use atomic force microscopy ( AFM) to quantify the adhesive and mechanical properties of individual ectoderm, mesoderm and endoderm progenitor cells from gastrulating zebrafish embryos. Combining these data with tissue self-assembly in vitro and the sorting behaviour of progenitors in vivo, we have shown that differential actomyosin-dependent cell-cortex tension, regulated by Nodal/ TGF beta-signalling ( transforming growth factor beta), constitutes a key factor that directs progenitor-cell sorting. These results demonstrate a previously unrecognized role for Nodal-controlled cell-cortex tension in germ-layer organization during gastrulation.
引用
收藏
页码:429 / U122
页数:19
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