CpG Oligodeoxynucleotides Induces Apoptosis of Human Bladder Cancer Cells via Caspase-3-Bax/Bcl-2-p53 Axis

被引：69

作者：

Luo, Yang ^{[1
]}

Fu, Xiaoyi ^{[1
]}

Ru, Ruizhen ^{[1
]}

Han, Bin ^{[1
]}

Zhang, Fafu ^{[1
]}

Yuan, Lihong ^{[1
]}

Men, Hongsheng ^{[2
]}

Zhang, Shulin ^{[3
]}

Tian, Sujuan ^{[1
]}

Dong, Bin ^{[1
]}

Meng, Minjie ^{[1
]}

机构：

[1] Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangzhou 510006, Peoples R China

[2] Univ Missouri, Rat Resource & Res Ctr, Dept Vet Pathobiol, Columbia, MO USA

[3] Shanghai Jiao Tong Univ, Sch Med, Dept Med Microbiol & Parasitol, Shanghai, Peoples R China

来源：

ARCHIVES OF MEDICAL RESEARCH | 2020年 / 51卷 / 03期

关键词：

CpG ODN; Human bladder cancer; Caspase-3; p53; Bax; Bcl-2; REGULATED GENE-EXPRESSION; SUPPRESSION; ACTIVATION; INHIBITOR; RECEPTOR; TUMOR; ODN; OLIGONUCLEOTIDES; ASSOCIATION; ADJUVANTS;

D O I：

10.1016/j.arcmed.2020.02.005

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Objective. To evaluate the anti-cancer effect of unmethylated cytosine-phosphorothioateguanine (CpG)-containing oligodeoxynucleotides (ODNs) on human bladder cancer UM-UC-3 cells, our study was carried out. Methods. The viability of cells (UM-UC-3, T24 and SV-HUC-1) with CpG ODN treatments was examined by cell counting kit-8 (CCK-8) assay. Apoptosis and cell cycle phase were determined by flow cytometry analysis. Pre-apoptosis factors of caspase-3, p53, B-cell lymphoma 2 associated X protein (Bax) and anti-apoptosis factor of B-cell lymphoma 2 (Bcl-2) were detected by western blot. Results. Experimental results showed that the viability of human bladder cancer cells (UM-UC-3 and T24) with CpG ODN treatment was decreased and the viability of human normal urothelial cells (SV-HUC-1) with CpG ODN treatment was increased with time-dependance manner. Moreover, CpG ODN increased the apoptosis rate of UM-UC-3 cells and arrested more cells in G0G1 phase. Furthermore, the expression of caspase-3, p53 and Bax were increased and the expression of Bcl-2 was decreased with CpG ODN treatment on UM-UC-3 cells. Conclusion. CpG ODN promoted the proliferation of normal urinary transitional epithelial cells (SV-HUC-1) and inhibited the cell viability of human bladder cancer cells (UM-UC-3 and T24) in vitro. CpG ODN induced the apoptosis of human bladder cancer (UM-UC-3) cells in a cascade progress via enhancing the expression of caspase-3, p53 and Bax, and inhibiting the expression of Bcl-2 with significant time-dependancy. CpG ODN inhibited cell cycle distribution of human bladder cancer (UM-UC-3) cells with more cells were arrested in G0G1 phase. This study suggested that the CpG ODN is the potential candidate on human bladder cancer. (C) 2020 IMSS. Published by Elsevier Inc.

引用

页码：233 / 244

页数：12

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