Separation of cephalosporin C acylase and penicillin V acylase from Aeromonas ACY 95 by chemical treatment

被引：4

作者：

Ambedkar, SS ^{[1
]}

Deshpande, BS ^{[1
]}

Shewale, JG ^{[1
]}

机构：

[1] HINDUSTAN ANTIBIOT LTD, RES & DEV, PUNE 411018, MAHARASHTRA, INDIA

来源：

PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 04期

关键词：

cephalosporin C acylase; penicillin V acylase; Aeromonas; 7-aminocephalosporanic acid; 6-aminopenicillanic acid; chemical extraction;

D O I：

10.1016/S0032-9592(96)00059-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several surfactants and solvents were evaluated for the extraction of cephalosporin C acylase from Aeromonas ACY 95. Treatment of the cells with acetone (0.5% v/v) allowed 85% recovery of cephalosporin C acylase with only 10% penicillin V acylase. (C) 1997 Elsevier Science Ltd.

引用

页码：305 / 307

页数：3

共 12 条

[1] BEIJERINCKIA-INDICA VAR PENICILLANICUM PENICILLIN-V ACYLASE - ENHANCED ENZYME-PRODUCTION BY CATABOLITE REPRESSION-RESISTANT MUTANT AND EFFECT OF SOLVENTS ON ENZYME-ACTIVITY [J].

AMBEDKAR, SS ;

DESHPANDE, BS ;

SUDHAKARAN, VK ;

SHEWALE, JG .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1991, 7 (03) :209-214

[2] CLONING AND NUCLEOTIDE SEQUENCING OF NEW GLUTARYL 7-ACA AND CEPHALOSPORIN-C ACYLASE GENES FROM PSEUDOMONAS STRAINS [J].

ARAMORI, I ;

FUKAGAWA, M ;

TSUMURA, M ;

IWAMI, M ;

ISOGAI, T ;

ONO, H ;

ISHITANI, Y ;

KOJO, H ;

KOHSAKA, M ;

UEDA, Y ;

IMANAKA, H .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 72 (04) :232-243

[3] ISOLATION OF SOIL STRAINS PRODUCING NEW CEPHALOSPORIN ACYLASES [J].

ARAMORI, I ;

FUKAGAWA, M ;

TSUMURA, M ;

IWAMI, M ;

YOKOTA, Y ;

KOJO, H ;

KOHSAKA, M ;

UEDA, Y ;

IMANAKA, H .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 72 (04) :227-231

[4] MOLECULAR-BIOLOGY OF BETA-LACTAM ACYLASES [J].

DESHPANDE, BS ;

AMBEDKAR, SS ;

SUDHAKARAN, VK ;

SHEWALE, JG .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1994, 10 (02) :129-138

[5] Cephalosporin C acylase and penicillin V acylase formation by Aeromonas sp ACY 95 [J].

Deshpande, BS ;

Ambedkar, SS ;

Shewale, JG .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (04) :373-378

[6]

DESHPANDE BS, Patent No. 386

[7]

LOWE DA, 1989, DEV IND MICROBIOL, V30, P121

[8]

Matsumoto K, 1993, Bioprocess Technol, V16, P67

[9]

SCHUTTE H, 1993, BIOTECHNOLOGY, V3, P505

[10]

SHEWALE JG, 1990, PROCESS BIOCHEM, V25, P97

← 1 2 →