Upconverting nanoparticles as nanotransducers for photodynamic therapy in cancer cells

被引：238

作者：

Chatterjee, Dev K. ^{[1
]}

Yong, Zhang ^{[2
]}

机构：

[1] Natl Univ Singapore, Div Bioengn, Singapore 117574, Singapore

[2] Natl Univ Singapore, Div Bioengn, Fac Engn, Singapore 117576, Singapore

来源：

NANOMEDICINE | 2008年 / 3卷 / 01期

关键词：

cancer; HT29 cell line; luminescence; photodynamic therapy; singlet oxygen; upconversion; wistar rats;

D O I：

10.2217/17435889.3.1.73

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Photodynamic therapy (PDT) involves killing of diseased cells by excitation of photosensitizer chemicals with high-energy light to produce cytotoxic oxygen species from surrounding dissolved oxygen. However, poor tissue penetration of high-energy light and hydrophobic photosensitizers limits the effectiveness to superficial pathologies. Upconversion phosphor nanoparticles convert low-energy radiation to higher-energy emissions. Aim: To create upconverting 'nanotransducers' to enable PDT in deep tissues. Results: Monodisperse, 50 nm PEI/NaYF4:Yb3+,Er3+ nanoparticles producing green/red emission on near-infrared (NIR) excitation were targeted to folate receptors on human colon cancer cells and imaged with high signal-to-background ratio. It was demonstrated that these particles could be excited after deep intramuscular injection in rats. On NIR excitation, the particles, modified with zinc phthalocyanin photosensitizer, released singlet oxygen and, after targeted binding to cancer cells, resulted in significant cell destruction. Conclusion: Potential clinical use of these nanoparticles includes imaging and PDT of cancer in deep tissues.

引用

页码：73 / 82

页数：10

共 24 条

[1] Upconversion fluorescence imaging of cells and small animals using lanthanide doped nanocrystals [J].

Chatteriee, Dev K. ;

Rufalhah, Abdul J. ;

Zhang, Yong .

BIOMATERIALS, 2008, 29 (07) :937-943

[2] Overexpression of folate binding protein α is one of the mechanism explaining the adaptation of HT29 cells to high concentration of methotrexate [J].

de Nonancourt-Didion, M ;

Guéant, JL ;

Adjalla, C ;

Chéry, C ;

Hatier, R ;

Namour, F .

CANCER LETTERS, 2001, 171 (02) :139-145

[3] Nanoparticles for two-photon photodynamic therapy in living cells [J].

Gao, De ;

Agayan, Rodney R. ;

Xu, Hao ;

Philbert, Martin A. ;

Kopelman, Raoul .

NANO LETTERS, 2006, 6 (11) :2383-2386

[4]

Grant WE, 1997, INT J CANCER, V71, P937, DOI 10.1002/(SICI)1097-0215(19970611)71:6<937::AID-IJC4>3.0.CO

[5]

2-Z

[6] Measuring the lifetime of singlet oxygen in a single cell: addressing the issue of cell viability [J].

Hatz, Sonja ;

Lambert, John D. C. ;

Ogilby, Peter R. .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2007, 6 (10) :1106-1116

[7] Highly efficient multicolour upconversion emission in transparent colloids of lanthanide-doped NaYF4 nanocrystals [J].

Heer, S ;

Kömpe, K ;

Güdel, HU ;

Haase, M .

ADVANCED MATERIALS, 2004, 16 (23-24) :2102-+

[8] The cost-effectiveness of Foscan mediated photodynamic therapy (Foscan-PDT) compared with extensive palliative surgery and palliative chemotherapy for patients with advanced head and neck cancer in the UK [J].

Hopper, C ;

Niziol, C ;

Sidhu, M .

ORAL ONCOLOGY, 2004, 40 (04) :372-382

[9] Cost-effectiveness of photodynamic therapy for treatment of Barrett's esophagus with high grade dysplasia [J].

Hur, C ;

Nishioka, NS ;

Gazelle, GS .

DIGESTIVE DISEASES AND SCIENCES, 2003, 48 (07) :1273-1283

[10] Photodynamic therapy for lung cancer - A review of 19 years' experience [J].

Kato, H .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1998, 42 (02) :96-99

← 1 2 3 →