Self-assembled composite matrix in a hierarchical 3-D scaffold for bone tissue engineering

被引：77

作者：

Chen, Muwan ^{[1
,2
,3
]}

Le, Dang Q. S. ^{[1
,2
]}

Baatrup, Anette ^{[1
]}

Nygaard, Jens V. ^{[2
]}

Hein, San ^{[2
]}

Bjerre, Lea ^{[1
]}

Kassem, Moustapha ^{[5
]}

Zou, Xuenong ^{[1
,4
]}

Buenger, Cody ^{[1
]}

机构：

[1] Aarhus Univ Hosp, Orthopaed Res Lab, DK-8000 Aarhus C, Denmark

[2] Univ Aarhus, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark

[3] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Pharm, Guangzhou 519000, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Orthopaed, Guangzhou 510275, Guangdong, Peoples R China

[5] Odense Univ Hosp, Dept Endocrinol & Metab, DK-5000 Odense C, Denmark

来源：

ACTA BIOMATERIALIA | 2011年 / 7卷 / 05期

关键词：

Human mesenchymal stem cell; Scaffold; Dynamic culture; Osteogenic differentiation; Real-time RT-PCR; MESENCHYMAL STEM-CELLS; FLOW PERFUSION CULTURE; MARROW STROMAL CELLS; IN-VITRO; EXTRACELLULAR MATRICES; SEEDING EFFICIENCY; DIFFERENTIATION; EXPRESSION; COLLAGEN; CARTILAGE;

D O I：

10.1016/j.actbio.2010.12.031

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

It is of high clinical relevance in bone tissue engineering that scaffolds promote a high seeding efficiency of cells capable of osteogenic differentiation, such as human bone marrow-derived mesenchymal stem cells (hMSCs) We evaluated the effects of a novel polycaprolactone (PCL) scaffold on hMSC seeding efficiency, proliferation, distribution and differentiation Porous PCL meshes prepared by fused deposition modeling (FDM) were embedded in matrix of hyaluronic acid, methylated collagen and terpolymer via polyelectrolyte complex coacervation Scaffolds were cultured statically and dynamically in osteogenic stimulation medium for up to 28 days. Compared to naked PCL scaffolds, embedded scaffolds provided a higher cell seeding efficiency (t-test, P < 0 05), a more homogeneous cell distribution and more osteogenically differentiated cells, verified by a more pronounced gene expression of the bone markers alkaline phosphatase, osteocalcin, bone sialoprotein I and bone sialoprotein II Dynamic culture resulted in higher amounts of DNA (day 14 and day 21) and calcium (day 21 and clay 28), compared to static culture Dynamic culture and the embedding synergistically enhanced the calcium deposition of hMSC on day 21 and day 28 This in vitro study provides evidence that hybrid scaffolds made from natural and synthetic polymers improve cellular seeding efficiency, proliferation, distribution and osteogenic differentiation (C) 2010 Acta Materialia Inc Published by Elsevier Ltd All rights reserved

引用

页码：2244 / 2255

页数：12

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