Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing

被引：44

作者：

Girish, A

Sun, HY

Yeo, DSY

Chen, GYJ

Chua, TK

Yao, SQ

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 10期

关键词：

intein; N-terminal cysteine-containing protein; protein array; proteomics;

D O I：

10.1016/j.bmcl.2005.03.079

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system. (c) 2005 Elsevier Ltd. All rights reserved.

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页码：2447 / 2451

页数：5

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