A procedure for the large-scale isolation of major bovine seminal plasma proteins

被引：64

作者：

Calvete, JJ

Varela, PF

Sanz, L

Romero, A

Mann, K

TopferPetersen, E

机构：

[1] CSIC, INST QUIM FIS ROCASOLANO, E-28006 MADRID, SPAIN

[2] MAX PLANCK INST BIOCHEM, D-82152 MARTINSRIED, GERMANY

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1996年 / 8卷 / 01期

关键词：

D O I：

10.1006/prep.1996.0073

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a common laboratory, and yields large amounts of pure proteins for biochemical and structural studies. (C) 1996 Academic Press, Inc.

引用

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页码：48 / 56

页数：9

相关论文

共 44 条

[1] RAPID CHARACTERIZATION OF ASPARAGINE-LINKED OLIGOSACCHARIDES ISOLATED FROM GLYCOPROTEINS USING A CARBOHYDRATE ANALYZER [J].

ANUMULA, KR ;

TAYLOR, PB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 195 (01) :269-280

[2] CRYSTAL-STRUCTURE OF A COMPLEX BETWEEN SERRATIA-MARCESCENS METALLOPROTEASE AND AN INHIBITOR FROM ERWINIA-CHRYSANTHEMI [J].

BAUMANN, U ;

BAUER, M ;

LETOFFE, S ;

DELEPELAIRE, P ;

WANDERSMAN, C .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 248 (03) :653-661

[3] A HELPING HAND FOR COLLAGENASES - THE HEMOPEXIN-LIKE DOMAIN [J].

BODE, W .

STRUCTURE, 1995, 3 (06) :527-530

[4] THE CUB DOMAIN - A WIDESPREAD MODULE IN DEVELOPMENTALLY-REGULATED PROTEINS [J].

BORK, P ;

BECKMANN, G .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (02) :539-545

[5] THE ANTITUMOR ACTION OF SEMINAL RIBONUCLEASE AND ITS QUATERNARY CONFORMATIONS [J].

CAFARO, V ;

DELORENZO, C ;

PICCOLI, R ;

BRACALE, A ;

MASTRONICOLA, MR ;

DIDONATO, A ;

DALESSIO, G .

FEBS LETTERS, 1995, 359 (01) :31-34

[6] LOCALIZATION AND STRUCTURAL CHARACTERIZATION OF AN OLIGOSACCHARIDE O-LINKED TO BOVINE PDC-109 - QUANTITATION OF THE GLYCOPROTEIN IN SEMINAL PLASMA AND ON THE SURFACE OF EJACULATED AND CAPACITATED SPERMATOZOA [J].

CALVETE, JJ ;

RAIDA, M ;

SANZ, L ;

WEMPE, F ;

SCHEIT, KH ;

ROMERO, A ;

TOPFERPETERSEN, E .

FEBS LETTERS, 1994, 350 (2-3) :203-206

[7] AMINO-ACID-SEQUENCE OF HSP-1, A MAJOR PROTEIN OF STALLION SEMINAL PLASMA - EFFECT OF GLYCOSYLATION ON ITS HEPARIN-BINDING AND GELATIN-BINDING CAPABILITIES [J].

CALVETE, JJ ;

MANN, K ;

SCHAFER, W ;

SANZ, L ;

REINERT, M ;

NESSAU, S ;

RAIDA, M ;

TOPFERPETERSEN, E .

BIOCHEMICAL JOURNAL, 1995, 310 :615-622

[8]

CALVETE JJ, 1995, FERTILITAT, V11, P35

[9] BUILDING PROTEINS WITH FIBRONECTIN TYPE-III MODULES [J].

CAMPBELL, ID ;

SPITZFADEN, C .

STRUCTURE, 1994, 2 (05) :333-337

[10] DETRIMENTAL EFFECT OF SEMINAL PLASMA ON THE FERTILIZING CAPACITY OF SPERM [J].

CHANG, MC .

NATURE, 1957, 179 (4553) :258-259

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