Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches

被引:3
作者
Fujimori, H
Miura, S
Koseki, S
Hokari, R
Tsuzuki, Y
Komoto, S
Hara, Y
Suzuki, H
Serizawa, H
Ishii, H
机构
[1] Natl Def Med Coll, Dept Internal Med 2, Tokorozawa, Saitama 3598513, Japan
[2] Keio Univ, Sch Med, Dept Internal Med, Tokyo, Japan
[3] Kitasato Inst Hosp, Dept Internal Med, Tokyo, Japan
关键词
CINC/gro; T lymphocytes; Peyer's patch; postcapillary venules; transendothelial migration; collecting lymphatics;
D O I
10.1159/000051919
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/Aims: Cytokine-induced neutrophil chemoattractant (CINC/gro), a member of interleukin-8 family, was found as a potent chemotactic factor for rat neutrophils. Although several chemokines have been shown to be potent regulators of T cell chemotaxis in vitro, the potential role of chemokines in T-cell migration in gut-associated lymphoid tissues has not been investigated in vivo. in the present study, the effects of CINC/gro on T-lymphocyte migration were examined in rat Peyer's patches. Methods: T lymphocytes collected from intestinal lymph of rats were fluorescence-labeled and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy and the effects of CINC/gro infusion was investigated. Lymphocyte flux in mesenteric collecting lymphatics was also observed. Results: In vivo infusion of CINC/gro significantly attenuated the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, once these lymphocytes adhered to venules, CINC/gro treatment significantly accelerated the transendothelial migration of T lymphocytes and they also significantly increased their subsequent flux in collecting lymphatics. Conclusion: There is a possibility that CINC/gro could modulate the characteristics of T lymphocyte homing in the inflammatory sites of gut. Copyright (C) 2001 S. Karger AG, Basel.
引用
收藏
页码:97 / 102
页数:6
相关论文
共 30 条
[21]  
SANTAMARIA BLF, 1996, EIR J IMMUNOL, V26, P2056
[22]   TRAFFIC SIGNALS ON ENDOTHELIUM FOR LYMPHOCYTE RECIRCULATION AND LEUKOCYTE EMIGRATION [J].
SPRINGER, TA .
ANNUAL REVIEW OF PHYSIOLOGY, 1995, 57 :827-872
[23]   RAT CINC/GRO - A NOVEL MEDIATOR FOR LOCOMOTIVE AND SECRETAGOGUE ACTIVATION OF NEUTROPHILS IN-VIVO [J].
SUZUKI, H ;
SUEMATSU, M ;
MIURA, S ;
LIU, YY ;
WATANABE, K ;
MIYASAKA, M ;
TSURUFUJI, S ;
TSUCHIYA, M .
JOURNAL OF LEUKOCYTE BIOLOGY, 1994, 55 (05) :652-657
[24]  
Tanabe S, 1997, J IMMUNOL, V159, P5671
[25]   T-CELL ADHESION INDUCED BY PROTEOGLYCAN-IMMOBILIZED CYTOKINE MIP-1-BETA [J].
TANAKA, Y ;
ADAMS, DH ;
HUBSCHER, S ;
HIRANO, H ;
SIEBENLIST, U ;
SHAW, S .
NATURE, 1993, 361 (6407) :79-82
[26]   alpha 4 integrin plays a critical role in early stages of T lymphocyte migration in Peyer's patches of rats [J].
Tsuzuki, Y ;
Miura, S ;
Suematsu, M ;
Kurose, I ;
Shigematsu, T ;
Kimura, H ;
Higuchi, H ;
Serizawa, H ;
Yagita, H ;
Okumura, K ;
Ishii, H .
INTERNATIONAL IMMUNOLOGY, 1996, 8 (03) :287-295
[27]  
VADDI K, 1994, J IMMUNOL, V153, P4721
[28]  
WALTZ A, 1987, BIOCHEM BIOPH RES CO, V149, P755
[29]   The role of chemokines in the micro environmental control of T versus B cell arrest in Peyer's patch high endothelial venules [J].
Warnock, RA ;
Campbell, JJ ;
Dorf, ME ;
Matsuzawa, A ;
McEvoy, LM ;
Butcher, EC .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (01) :77-88
[30]   PURIFICATION AND CHARACTERIZATION OF CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT PRODUCED BY EPITHELIOID CELL-LINE OF NORMAL RAT-KIDNEY (NRK-52E CELL) [J].
WATANABE, K ;
KINOSHITA, S ;
NAKAGAWA, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (03) :1093-1099