Phosphorylation-dependent and -independent functions of p130 cooperate to evoke a sustained G1 block

The retinoblastoma (pRb)-related p130 pocket protein is a regulator of cell growth and differentiation, and a candidate tumour suppressor. Both pRb and p130 operate through interactions with cellular proteins, including the E2F transcription factors. While such interactions are controlled by phosphorylation of multiple sites of pRb, regulation of p130 remains poorly understood. We now identify 22 in vivo phosphorylation sites of p130, targeted by diverse kinases, and present evidence for three cyclin-dependent kinase 4(6) [Cdk4(6)] specific phosphorylations, which appear critical for controlling the growth-restraining activity of p130, When expressed in U20S cells, the phosphorylation-deficient mutant p130(Delta Cdk4), in which the Cdk4 specific sites were mutated to alanine residues, imposed a more sustained G(1) arrest than a constitutively active pRb(Delta Cdk), known to repress all cellular E2F activity. Experiments using p130(Delta Cdk4).,d another phosphorylation-deficient mutant, p130(PM19A) with 19 phosphorylation sites mutated, revealed that the p130-imposed G(1) block reflects cooperative growth-suppressive effects of phosphorylation-regulated E2F binding and phosphorylation-independent sequestration of cyclin E(A)-Cdk2 through the N-terminal cyclin binding motif of p130.