Effect of enzyme extracts isolated from white-rot fungi on chemical composition and in vitro digestibility of wheat straw

被引：85

作者：

Rodrigues, M. A. M. ^{[1
]}

Pinto, P. ^{[2
]}

Bezerra, R. M. F. ^{[2
]}

Dias, A. A. ^{[2
]}

Guedes, C. V. M. ^{[1
]}

Cardoso, V. M. G. ^{[1
]}

Cone, J. W. ^{[3
]}

Ferreira, L. M. M. ^{[1
]}

Colaco, J. ^{[1
]}

Sequeira, C. A. ^{[1
]}

机构：

[1] Univ Tras Os Montes & Alto Douro, CECAV, Dept Anim Sci, P-5001801 Vila Real, Portugal

[2] Univ Tras Os Montes & Alto Douro, CETAV, Dept Biol & Environm Engn, P-5001801 Vila Real, Portugal

[3] Wageningen Univ, Dept Anim Sci, Anim Nutr Grp, Wageningen, Netherlands

来源：

ANIMAL FEED SCIENCE AND TECHNOLOGY | 2008年 / 141卷 / 3-4期

关键词：

white-rot fungi; in vitro digestibility; wheat straw;

D O I：

10.1016/j.anifeedsci.2007.06.015

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A series of in vitro experiments were completed to evaluate the potential of enzyme extracts, obtained from the white-rot fungi Trametes versicolor (TV1, TV2), Bjerkandera adusta (BA) and Fomes fomentarius (FF), to increase degradation of cell wall components of wheat straw. The studies were conducted as a completely randomized design and analysed using one-way ANOVA. Enzyme activities of the extracts, previously obtained from a liquid culture medium, were characterized in terms of laccase and peroxidase for ligninolytic activity. Carboxymethyl cellulase (CMCase) and avicell digesting cellulase (Avicelase) were used for cellulolytic enzyme assays. Wheat straw samples were incubated with enzyme extracts in a citrate buffer (pH 5.0) in a forced air oven at 25 degrees C for 6 days. In vitro NDF digestibility (IVNDFD), and the rate and extent of NDF fermentation, without and after incubation with the white-rot enzyme extracts, were determined using a gravimetric microbiological method and a gas production technique, respectively. Results from cell wall chemical composition showed that TV2 and BA enzyme extracts decreased NDF concentration (P<0.05) and that TV1 had higher activity (P<0.05) towards cellulose. There was an increase in IVNDFD (P<0.05), resulting from treatment of wheat straw with enzyme extracts from BA, TV1 and TV2, reaching a difference of 13% for TV2 (P<0.05), versus the non-treated straw control. Treatment with enzyme extract from TV2 caused increased gas production (P<0.05) after the first 20 h of incubation, and also increased the maximum rate of gas production, thus enhancing fermentation kinetics. This study indicates that enzyme extracts from white-rot fungi can be used to develop new approaches to overcome low digestibility of some plant cell walls. Utilization of different substrates to produce enzyme extracts can lead to production of viable ligninolytic complexes which could improve the nutritive value of fibrous feeds. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：326 / 338

页数：13

共 45 条

[11] Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus [J].

Cone, JW ;

vanGelder, AH ;

Visscher, GJW ;

Oudshoorn, L .

ANIMAL FEED SCIENCE AND TECHNOLOGY, 1996, 61 (1-4) :113-128

[12] USE OF PIVALIC ACID AS A REFERENCE SUBSTANCE IN MEASUREMENTS OF PRODUCTION OF VOLATILE FATTY-ACIDS BY RUMEN MICROORGANISMS INVITRO [J].

CZERKAWSKI, JW .

BRITISH JOURNAL OF NUTRITION, 1976, 36 (02) :311-315

[13] Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides [J].

de Vries, RP ;

Kester, HCM ;

Poulsen, CH ;

Benen, JAE ;

Visser, J .

CARBOHYDRATE RESEARCH, 2000, 327 (04) :401-410

[14] In vivo and laccase-catalysed decolourization of xenobiotic azo dyes by a basidiomycetous fungus:: characterization of its ligninolytic system [J].

Dias, AA ;

Bezerra, RM ;

Lemos, PM ;

Pereira, AN .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2003, 19 (09) :969-975

[15]

Gangwar M, 2003, INDIAN J ANIM SCI, V73, P87

[16]

Goering H.K., 1970, FORAGE FIBER ANAL AP, V379

[17]

GOMES VA, 2004, THESIS U TRAS OS MON

[18] Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds [J].

Groot, JCJ ;

Cone, JW ;

Williams, BA ;

Debersaques, FMA ;

Lantinga, EA .

ANIMAL FEED SCIENCE AND TECHNOLOGY, 1996, 64 (01) :77-89

[19]

Jalc D, 2002, MYCOTA, V11, P19

[20] Effect of white-rot basidiomycetes-treated wheat straw on rumen fermentation in artificial rumen [J].

Jalc, D ;

Nerud, F ;

Erbanova, P ;

Siroka, P .

REPRODUCTION NUTRITION DEVELOPMENT, 1996, 36 (03) :263-270

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