Complete nucleotide sequences of two RNA segments of human picobirnavirus

被引：77

作者：

Wakuda, M

Pongsuwanna, Y

Taniguchi, K ^{[1
]}

机构：

[1] Fujita Hlth Univ, Sch Med, Dept Virol & Parasitol, Toyoake, Aichi 4701192, Japan

[2] Natl Inst Hlth, Dept Med Sci, Enter & Resp Viruses Lab, Nonthaburi 110001, India

来源：

JOURNAL OF VIROLOGICAL METHODS | 2005年 / 126卷 / 1-2期

关键词：

picobirnavirus; gastroenteritis; nucleotide sequence; double-stranded RNA;

D O I：

10.1016/j.jviromet.2005.02.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Picobirnaviruses are unclassified, non-enveloped, spherical, small viruses with a genome comprising two double-stranded RNA segments. Only incomplete sequence data on picobirnaviruses are available so far. By cloning involving single primer amplification, full-length cDNAs were prepared corresponding to RNA segments 1 and 2 of a picobirnavirus (strain Hy005 102) isolated from a stool specimen from an infant with acute non-bacterial gastroenteritis in Thailand, and the complete nucleotide sequences were determined. RNA segments 1 and 2 are 2,525 and 1,745 base pairs in length, respectively. RNA segment I encodes two open reading frames (ORFs) of 224 and 552 amino acids, and RNA segment 2 codes for a single ORF of 534 amino acids. On comparison with a part of the nucleotide sequences of the RNA segment, 2 of the other published picobirnavirus strains, the Thai strain was found to be related most closely to one of the US strains. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：165 / 169

页数：5

共 26 条

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