Direct measurement of the adhesive strength of biofilms in pipes by micromanipulation

被引：87

作者：

Chen, MJ ^{[1
]}

Zhang, Z ^{[1
]}

Bott, TR ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem Engn, Ctr Bioproc Engn, Birmingham B15 2TT, W Midlands, England

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 12期

关键词：

biofilm; adhesive strength; Pseudomonas fluorescens biofilms; micromanipulation;

D O I：

10.1023/A:1008805326385

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel micromanipulation technique has been developed to measure directly the adhesive strength of biofilms formed in pipe flows. A T-shaped probe was specially designed to pull Pseudomonas fluorescens biofilms away from the inner surface of a pipe to which they were attached. The adhesive strength between biofilms and the substratum was defined as the work required to remove the biofilms per unit area from the substratum. Results showed that the adhesive strength increased with the fluid velocity in which the biofilms were grown, with a typical value of 0.05 similar to 0.2 J/m(2).

引用

页码：875 / 880

页数：6

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