Amperometric biosensor for tyrosinase inhibitors in a pure organic phase

被引:36
作者
Deng, Q [1 ]
Dong, SJ [1 ]
机构
[1] CHINESE ACAD SCI,CHANGCHUN INST APPL CHEM,ELECTROANALYT CHEM LAB,CHANGCHUN 130022,PEOPLES R CHINA
关键词
biosensor; amperometry; tyrosinase inhibitors; organic solvent; enzyme kinetics;
D O I
10.1039/an9962101979
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The determination of benzoic acid, thiourea and 2-mercaptoethanol in three pure organic solvents, viz., chloroform, chlorobenzene and 1,2-dichlorobenzene, by using an amperometric cryohydrogel tyrosinase biosensor is described. Measurements were carried out with phenol as the enzyme substrate. Kinetic parameters (K-i and I-50) were determined in the three solvents for various inhibitors. The sensor showed the most sensitive measurements to these inhibitors in pure chloroform. The solvent-induced deviation of the biosensor to thiourea was evaluated by means of Hill coefficients. The smallest deviation as observed in 1,2-dichlorobenzene, owing to the high hydrophobicity of this solvent. The nature of the inhibition process and its reversibility mere also examined.
引用
收藏
页码:1979 / 1982
页数:4
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