Effects of Helicobacter pylori infection on the link between regenerating gene expression and serum gastrin levels in mongolian gerbils

被引:43
作者
Fukui, H
Franceschi, F
Penland, RL
Sakai, T
Sepulveda, AR
Fujimori, T
Terano, A
Chiba, T
Genta, RM
机构
[1] Kyoto Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, Sakyo Ku, Kyoto 6068507, Japan
[2] Dokkyo Univ, Sch Med, Dept Pathol, Mibu, Tochigi, Japan
[3] Dokkyo Univ, Sch Med, Dept Gastroenterol, Mibu, Tochigi, Japan
[4] Baylor Coll Med, Houston, TX 77030 USA
[5] Vet Affairs Med Ctr, Dept Pathol Med & Microbiol & Immunol, Houston, TX 77030 USA
关键词
D O I
10.1097/01.LAB.0000106501.56339.CE
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Although regenerating gene (Reg) protein is reported to have atrophic effect on gastric epithelial cells, its involvement in human gastric diseases is not clear, We have recently shown that both gastrin and gastric mucosal inflammation enhance Reg gene expression in the fundic mucosa in rats. This study was designed to clarify whether Reg protein is involved in Helicobacter pylori-induced gastritis and whether Reg gene expression is linked to serum gastrin levels in this condition. Mongolian gerbils were inoculated with an H. pylori strain isolated from a gastric cancer patient. Four weeks later, some of the gerbils with H. pylori infection were eradicated by lansoprazole, amoxicillin, and clarithromycin. The time courses of changes in Reg gene expression, serum gastrin levels, gastric acidity, and histopathologic factors were examined. Four weeks after H. pylori infection, gastritis started spreading to the fundic mucosa, and gastric acidity started reducing. Serum gastrin levels and Reg mRNA expression in the fundus were significantly increased 6 weeks after infection. Reg mRNA expression in the fundus correlated significantly with both serum gastrin levels and the severity of fundic mucosal inflammation. After H. pylori eradication, serum gastrin levels and fundic mucosal inflammation were normalized, and the increase in Reg mRNA expression was abolished. The Reg gene is associated with hypergastrinemia and fundic mucosal inflammation and may be involved in H. pylori-induced gastritis.
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收藏
页码:1777 / 1786
页数:10
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