A method for synthesis of an artificial ribonuclease

被引：14

作者：

Åström, H ^{[1
]}

Strömberg, R ^{[1
]}

机构：

[1] Karolinska Inst, Scheele Lab, MBB, Div Organ & Bioorgan Chem, S-17177 Stockholm, Sweden

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002561

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-Amino-2,9-dimethyl-1,10-phenanthroline-oligonucleotide conjugates have been synthesized. A 2'-O-methyl octaribonucleotide carrying a 2'-aminoethoxymethyl linker in a central position was produced. Reaction of the aminoneocuproine phenyl carbamate with the fully deprotected oligonucleotide in aqueous solution gave virtually quantitative conversion into the conjugate. Preliminary cleavage studies in presence of zinc ions show nuclease activity towards RNA targets.

引用

页码：1385 / 1388

页数：4

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