Acute osmotic/stress stimuli induce a transient decrease of transcriptional activity in the neurosecretory neurons of supraoptic nuclei

被引:50
作者
Lafarga, M [1 ]
Berciano, MT
Garcia-Segura, LM
Andres, MA
Carmo-Fonseca, M
机构
[1] Univ Cantabria, Fac Med, Dept Anat & Cell Biol, Santander 39011, Spain
[2] CSIC, Inst Cajal, Madrid 28006, Spain
[3] Univ Lisbon, Fac Med, Inst Histol & Embryol, P-1699 Lisbon, Portugal
来源
JOURNAL OF NEUROCYTOLOGY | 1998年 / 27卷 / 04期
关键词
D O I
10.1023/A:1006937032068
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Administration of hypertonic NaCl solutions by intraperitoneal injection evokes a transient expression of immediate-early genes in the hypothalamic magnocellular neurons of supraoptic nuclei (SON), which is followed by an upregulation of arginine vasopressin synthesis and a general increase in cellular metabolic activity. Here we have analysed the changes that occur in the nucleus of SON neurons during the period of transient Fos expression after injection of hypertonic saline. Within the first 30 minutes after injection, the nuclei become significantly smaller, contain more condensed chromatin and incorporate less H-3-uridine than the controls. By 12 hours these effects are reverting and at 24 hours the nuclei are already more active than the controls. Additionally, we observe an initial decrease in the number of coiled bodies per nucleus within the first 2 hours, followed by a 3-fold increase at 24 hows after injection. As coiled bodies are transcription-dependent subnuclear 'organelles', these results further support the view that injection of hypertonic saline causes a transient inhibition of nuclear activity. Our data show that SON neurons respond to acute osmotic/stress stimuli first with inhibition and then with activation of gene expression. Importantly, inhibition of transcriptional activity occurs simultaneously with maximal accumulation of Fos protein in the nucleus, raising the possibility that activation of c-fos expression may cause repression of target genes.
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页码:205 / 217
页数:13
相关论文
共 46 条
[1]   HUMAN AUTOANTIBODY TO A NOVEL PROTEIN OF THE NUCLEAR COILED BODY - IMMUNOLOGICAL CHARACTERIZATION AND CDNA CLONING OF P80-COILIN [J].
ANDRADE, LEC ;
CHAN, EKL ;
RASKA, I ;
PEEBLES, CL ;
ROOS, G ;
TAN, EM .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (06) :1407-1419
[2]   THE HEAT-SHOCK RESPONSE IN HELA-CELLS IS ACCOMPANIED BY ELEVATED EXPRESSION OF THE C-FOS PROTOONCOGENE [J].
ANDREWS, GK ;
HARDING, MA ;
CALVET, JP ;
ADAMSON, ED .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (10) :3452-3458
[3]  
ANGEL PE, 1994, FOS JUN FAMILIES TRA, P310
[4]   RAPID MORPHOLOGICAL-CHANGES IN SUPRAOPTIC NUCLEUS AND POSTERIOR PITUITARY INDUCED BY A SINGLE HYPERTONIC SALINE INJECTION [J].
BEAGLEY, GH ;
HATTON, GI .
BRAIN RESEARCH BULLETIN, 1992, 28 (04) :613-618
[5]   Dispersion and culture of magnocellular neurons from the supraoptic nucleus of the adult rat [J].
Bilinski, M ;
Sanchez, A ;
Nicolini, VG ;
Villar, MJ ;
Tramezzani, JH .
JOURNAL OF NEUROSCIENCE METHODS, 1996, 64 (01) :13-18
[6]   MUTATIONAL ANALYSIS OF P80 COILIN INDICATES A FUNCTIONAL INTERACTION BETWEEN COILED BODIES AND THE NUCLEOLUS [J].
BOHMANN, K ;
FERREIRA, JA ;
LAMOND, AI .
JOURNAL OF CELL BIOLOGY, 1995, 131 (04) :817-831
[7]  
Bohmann K, 1995, J CELL SCI, P107
[8]  
BROADWELL RD, 1981, J CELL BIOL, V90, P474, DOI 10.1083/jcb.90.2.474
[9]  
BUKH A, 1990, J IMMUNOL, V144, P4835
[10]  
Cajal SRy, 1910, TRAB LAB INVEST BIOL, V8, P27