The influence of water activity on the enantioselectivity in the enzyme-catalyzed reduction of 2-pentanone

被引：16

作者：

Jonsson, A ^{[1
]}

van Breukelen, W ^{[1
]}

Wehtje, E ^{[1
]}

Adlercreutz, P ^{[1
]}

Mattiasson, B ^{[1
]}

机构：

[1] Univ Lund, Dept Biotechnol, Ctr Chem & Chem Engn, S-22100 Lund, Sweden

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1998年 / 5卷 / 1-4期

关键词：

organic medium; water activity; temperature; enantioselectivity; alcohol dehydrogenase; Thermoanaerobium brockii;

D O I：

10.1016/S1381-1177(98)00048-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stereoselective reduction of 2-pentanone by alcohol dehydrogenase from Thermoanaerobium brockii was studied at controlled water activity and at different reaction temperatures. The reaction rate increased when water activity was increased from 0.32 to 0.96 and when raising the temperature from 5 degrees C to 40 degrees C. The enantioselectivity, E, reached a plateau value at high water activities. The enantioselectivity increased with decreasing reaction temperature. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：273 / 276

页数：4

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