A hyperspectral fluorescence lifetime probe for skin cancer diagnosis

被引：73

作者：

De Beule, P. A. A. ^{[1
]}

Dunsby, C. ^{[1
]}

Galletly, N. P. ^{[2
]}

Stamp, G. W. ^{[2
]}

Chu, A. C. ^{[3
]}

Anand, U. ^{[4
]}

Anand, P. ^{[4
]}

Benham, C. D. ^{[5
]}

Naylor, A. ^{[5
]}

French, P. M. W. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Histopathol, London W12 0NN, England

[3] Univ London Imperial Coll Sci Technol & Med, Dept Dermatol, London W12 0NN, England

[4] Univ London Imperial Coll Sci Technol & Med, Dept Neurosci & Mental Hlth, London W12 0NN, England

[5] GlaxoSmithKline, Harlow CM19 5AW, Essex, England

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2007年 / 78卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1063/1.2818785

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The autofluorescence of biological tissue can be exploited for the detection and diagnosis of disease but, to date, its complex nature and relatively weak signal levels have impeded its widespread application in biology and medicine. We present here a portable instrument designed for the in situ simultaneous measurement of autofluorescence emission spectra and temporal decay profiles, permitting the analysis of complex fluorescence signals. This hyperspectral fluorescence lifetime probe utilizes two ultrafast lasers operating at 355 and 440 nm that can excite autofluorescence from many different biomolecules present in skin tissue including keratin, collagen, nicotinamide adenine dinucleotide (phosphate), and flavins. The instrument incorporates an optical fiber probe to provide sample illumination and fluorescence collection over a millimeter-sized area. We present a description of the system, including spectral and temporal characterizations, and report the preliminary application of this instrument to a study of recently resected (<2 h) ex vivo skin lesions, illustrating its potential for skin cancer detection and diagnosis. (C) 2007 American Institute of Physics.

引用

页数：7

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