Roles of 5-substituents of tRNA wobble uridines in the recognition of purine-ending codons

被引:81
作者
Takai, K
Yokoyama, S
机构
[1] Ehime Univ, Cell Free Sci & Technol Res Ctr, Matsuyama, Ehime 7908577, Japan
[2] Ehime Univ, Fac Engn, Dept Appl Chem, Matsuyama, Ehime 7908577, Japan
[3] Ehime Univ, Venture Business Lab, Matsuyama, Ehime 7908577, Japan
[4] Univ Tokyo, Sch Sci, Dept Biochem & Biophys, Bunkyo Ku, Tokyo 1130033, Japan
[5] RIKEN, Genom Sci Ctr, Prot Res Grp, Yokohama, Kanagawa 2300045, Japan
[6] RIKEN, Harima Inst Spring 8, Cellular Signaling Lab, Sayo, Hyogo 6795148, Japan
[7] RIKEN, Harima Inst Spring 8, Structurome Res Grp, Sayo, Hyogo 6795148, Japan
关键词
D O I
10.1093/nar/gkg839
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many tRNA molecules that recognize the purine-ending codons but not the pyrimidine-ending codons have a modified uridine at the wobble position, in which a methylene carbon is attached directly to position 5 of the uracil ring. Although several models have been proposed concerning the mechanism by which the 5-substituents regulate codon-reading properties of the tRNAs, none could explain recent results of the experiments utilizing well-characterized modification-deficient strains of Escherichia coli. Here, we first summarize previous studies on the codon-reading properties of tRNA molecules with a U derivative at the wobble position. Then, we propose a hypothetical mechanism of the reading of the G-ending codons by such tRNA molecules that could explain the experimental results. The hypothesis supposes unconventional base pairs between a protonated form of the modified uridines and the G at the third position of the codon stabilized by two direct hydrogen bonds between the bases. The hypothesis also addresses differences between the prokaryotic and eukaryotic decoding systems.
引用
收藏
页码:6383 / 6391
页数:9
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