Modified αA crystallin in the retina:: Altered expression and truncation with aging

被引:52
作者
Kapphahn, RJ
Ethen, CM
Peters, EA
Higgins, L
Ferrington, DA
机构
[1] Univ Minnesota, Dept Ophthalmol, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Biochem, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Mol Biol & Biophys, Minneapolis, MN 55455 USA
关键词
D O I
10.1021/bi034774e
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crystallins are small heat shock proteins with chaperone function that prevent heat- and oxidative stress-induced aggregation of proteins. This is the first report describing modifications of alphaA crystallin in the sensory retina, including altered content and truncation with aging. Proteins from adult, middle age, and old Fischer 344 Brown Norway rats were compared. Western immunoblotting was used to evaluate alphaA crystallin content and identify protein spots on two-dimensional gels containing alphaA crystallin. The type and site of multiple post-translational modifications were identified by mass spectrometry. We found the content of a-A crystallin was significantly decreased in the oldest rats. On two-dimensional gels, retinal crystallins resolved into multiple spots with altered migration, indicative of changes in intrinsic charge and/or truncation. Post-translational modifications that were identified included oxidation, phosphorylation, deamidation, acetylation, and truncation. In samples from rats of all ages, a highly modified N-terminus containing these modifications was found. We also observed an age-dependent difference in the extent of N- and C-terminal truncation. These results suggest that protection against stress-induced protein aggregation is compromised in the aged retina.
引用
收藏
页码:15310 / 15325
页数:16
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