Horizontal transfer of the gene encoding outer membrane protein P2 of nontypeable Haemophilus influenzae, in a patient with chronic obstructive pulmonary disease

被引:22
作者
Hiltke, TJ
Schiffmacher, AT
Dagonese, AJ
Sethi, S
Murphy, TF
机构
[1] Vet Affairs Western New York Healthcare Syst, Buffalo VAMC, Buffalo, NY 14215 USA
[2] SUNY Buffalo, Dept Med, Div Infect Dis, Buffalo, NY 14260 USA
[3] SUNY Buffalo, Dept Med, Div Pulm & Crit Care Med, Buffalo, NY 14260 USA
[4] SUNY Buffalo, Dept Microbiol, Buffalo, NY 14260 USA
关键词
D O I
10.1086/375724
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An adult with chronic obstructive pulmonary disease was monitored prospectively for 2 years. Nontypeable Haemophilus influenzae was isolated from sputum cultures at 22 of 23 monthly clinic visits. Analysis of the isolates, by pulsed-field gel electrophoresis (PFGE), revealed that the patient was colonized by 3 different strains during the 2-year period. The gene encoding outer-membrane protein (OMP) P2, ompP2, was amplified from sputum samples and selected strains obtained from this patient. Analysis of the ompP2 sequences, in combination with the PFGE patterns, indicated that ompP2 horizontal transfer between 2 strains occurred in the respiratory tract, between clinic visits 13 and 14. Observation of ompP2 horizontal transfer in the human respiratory tract has important implications for both the understanding of ompP2 diversity among strains and the future design of OMP P2-based vaccines.
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收藏
页码:114 / 117
页数:4
相关论文
共 13 条
[1]   DIVERSITY OF THE P2 PROTEIN AMONG NONTYPABLE HAEMOPHILUS-INFLUENZAE ISOLATES [J].
BELL, J ;
GRASS, S ;
JEANTEUR, D ;
MUNSON, RS .
INFECTION AND IMMUNITY, 1994, 62 (06) :2639-2643
[2]   Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates [J].
Benson, JA ;
Ferrieri, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (08) :3006-3008
[3]   ANTIGENIC DRIFT OF NONENCAPSULATED HAEMOPHILUS-INFLUENZAE MAJOR OUTER-MEMBRANE PROTEIN P2 IN PATIENTS WITH CHRONIC-BRONCHITIS IS CAUSED BY POINT MUTATIONS [J].
DUIM, B ;
VANALPHEN, L ;
EIJK, P ;
JANSEN, HM ;
DANKERT, J .
MOLECULAR MICROBIOLOGY, 1994, 11 (06) :1181-1189
[4]   Antigenic diversity and gene polymorphisms in Haemophilus influenzae [J].
Gilsdorf, JR .
INFECTION AND IMMUNITY, 1998, 66 (11) :5053-5059
[5]   Sequence stability of the gene encoding outer membrane protein P2 of nontypeable Haemophilus influenzae in the human respiratory tract [J].
Hiltke, TJ ;
Sethi, S ;
Murphy, TF .
JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (05) :627-631
[6]   The burden of otitis media [J].
Klein, JO .
VACCINE, 2000, 19 :S2-S8
[7]   Simultaneous respiratory tract colonization by multiple strains of nontypeable Haemophilus influenzae in chronic obstructive pulmonary disease:: Implications for antibiotic therapy [J].
Murphy, TF ;
Sethi, S ;
Klingman, KL ;
Brueggemann, AB ;
Doern, GV .
JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (02) :404-409
[8]   New strains of bacteria and exacerbations of chronic obstructive pulmonary disease [J].
Sethi, S ;
Evans, N ;
Grant, BJB ;
Murphy, TF .
NEW ENGLAND JOURNAL OF MEDICINE, 2002, 347 (07) :465-471
[9]   Airway inflammation and etiology of acute exacerbations of chronic bronchitis [J].
Sethi, S ;
Muscarella, K ;
Evans, N ;
Klingman, KL ;
Grant, BJB ;
Murphy, TF .
CHEST, 2000, 118 (06) :1557-1565
[10]   Bacterial infection in chronic obstructive pulmonary disease in 2000: a state-of-the-art review [J].
Sethi, S ;
Murphy, TF .
CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (02) :336-+