An approach to speed up the isolation of hydrophilic metabolites from natural sources at semipreparative level by using a hydrophilic-lipophilic balance/mixed-mode strong cation exchange-high-performance liquid chromatography/mass spectrometry system

被引：18

作者：

Espada, Alfonso ^{[1
]}

Anta, Cristina ^{[1
]}

Bragado, Aroa ^{[1
]}

Rodriguez, Jaime ^{[2
]}

Jimenez, Carlos ^{[2
]}

机构：

[1] Lilly SA, Analyt Technol DCR&T Alcobendas, Alcobendas Madrid 28108, Spain

[2] Univ A Coruna, Fac Ciencias, Dept Quim Fundamental, La Coruna 15071, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 13期

关键词：

Hydrophilic-lipophilic balance (HLB); Mixed-mode strong cation exchange (MCX); SPE; HPLC/MS; Small water-soluble compounds; Natural products; Siderophore; VIBRIO-ANGUILLARUM STRAINS; MASS-SPECTROMETRY; ENVIRONMENTAL WATERS; CLIONA-TENUIS; PRODUCTS; VANCHROBACTIN;

D O I：

10.1016/j.chroma.2011.01.072

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

An approach to speed up the isolation of hydrophilic metabolites in complex natural matrixes by using a HLB/MCX-HPLC/MS system based on the retention properties of hydrophilic-lipophilic and cation exchange polymeric cartridges was developed. This methodology was successfully applied to the reisolation of small water soluble compounds with completely different structures from two different natural extracts such as a dipeptide (vanchrobactin) from a bacterium culture broth and a pyrrolidine bearing a carboxylic acid moiety (clionapyrrolidine A) from a sponge. This method improved not only the efficiency of the isolation methodology but also the isolation time in relation to the existing methods. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1790 / 1794

页数：5

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