Inflorescences of Brassicacea species as source of bioactive compounds: A comparative study

被引:47
作者
Sousa, Carla [1 ]
Taveira, Marcos [1 ]
Valentao, Patricia [1 ]
Fernandes, Fatima [1 ]
Pereira, Jose A. [2 ]
Estevinho, Leticia [2 ]
Bento, Albino [2 ]
Ferreres, Federico [3 ]
Seabra, Rosa M. [1 ]
Andrade, Paula B. [1 ]
机构
[1] Univ Porto, Fac Farm, Serv Farmacognosia, REQUIMTE, P-4050047 Oporto, Portugal
[2] Escola Super Agr, Inst Politecn Braganca, CIMO, P-5301855 Braganca, Portugal
[3] CEBAS CSIC, Dept Food Sci & Technol, Res Grp Qual Safety & Bioact Plant Foods, Murcia 30100, Spain
关键词
Brassica oleracea L. var. costata DC; Brassica oleracea L. var. acephala; Brassica rapa L. var. rapa; inflorescences; phenolic compounds; organic acids; antioxidant activity; antimicrobial activity;
D O I
10.1016/j.foodchem.2008.02.087
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Two Brassica oleracea varieties (B. oleracea L. var. costata DC and B. oleracea L. var. acephala) and Brassica rapa L. var. rapa inflorescences were studied for their chemical composition and antioxidant capacity. Phenolic compounds and organic acids profiles were determined by HPLC-DAD and HPLC-UV, respectively. B. oleracea var. costata and B. oleracea L. var. acephala inflorescences presented a similar qualitative phenolic composition, exhibiting several complex kaempferol derivatives and 3-p-coumaroylquinic acid, while B. rapa var. rapa was characterized by kaempferol and isorhamnetin glycosides and several phenolic acids derivatives. B. oleracea L. var. costata and B. rapa var. rapa showed the highest phenolics content. The three Brassica exhibited the same six organic acids (aconitic, citric, pyruvic, malic, shikimic and fumaric acids), but B. oleracea L. var. acephala presented a considerably higher amount. Each inflorescence was investigated for its capacity to act as a scavenger of DPPH radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration dependent manner against all radicals. These samples were also studied for its antimicrobial potential against Gram-positive and Gram-negative bacteria and fungi, displaying antimicrobial capacity only against Gram-positive bacteria. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:953 / 961
页数:9
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