Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling

被引：63

作者：

Akimoto, T

Ushida, T

Miyaki, S

Akaogi, H

Tsuchlya, K

Yan, Z

Williams, RS

Tateishi, T

机构：

[1] Univ Tokyo, Dept Life Sci, Grad Sch Arts & Sci, Tokyo 1538902, Japan

[2] Univ Tokyo, Div Biomed Mat & Syst, Ctr Dis Biol & Integrat Med, Grad Sch Med, Tokyo 1130033, Japan

[3] Natl Inst Mat Sci, Ctr Biomat, Tsukuba, Ibaraki 3050044, Japan

[4] Univ Tsukuba, Dept Orthoped, Inst Clin Med, Tsukuba, Ibaraki 3058575, Japan

[5] Duke Univ, Med Ctr, Div Cardiol, Dept Med, Durham, NC 27704 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 329卷 / 01期

关键词：

mechanical stress; mechanical stretch; trans-differentiation; C2C12; Wnt10; myoblasts; adipocytes;

D O I：

10.1016/j.bbrc.2005.01.136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myoblasts are able to differentiate into other mesenchymal lineages including adipocytes and osteoblasts. However, it is not known how this differentiation is normally regulated in intact animals and humans. Here, we subjected cultured C2CI2 myoblasts to cyclic mechanical stretch (20% elongation) during differentiation into adipocytes. Mechanical stretch inhibited the myoblast-toadipocyte differentiation significantly, concurrent with an enhanced expression of WntlOb mRNA. Inhibition of the Writ signaling by incubation of the myoblasts with a soluble Writ ligand, sFRP-2, abolished the inhibitory function of mechanical stretch on adipogenesis. These findings provide evidence that mechanical stretch inhibits myoblast-to-adipocyte differentiation possibly through Writ signaling. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：381 / 385

页数：5

共 22 条

[1] Mechanical stretch is a down-regulatory signal for differentiation of C-2C12 myogenic cells [J].