Colorimetric method for the determination of lipoxygenase activity

被引：94

作者：

Anthon, GE ^{[1
]}

Barrett, DM ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 01期

关键词：

lipoxygenase; colorimetric assay; rapid; vegetable homogenates; hemoglobin;

D O I：

10.1021/jf000871s

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A colorimetic assay for lipoxygenase activity has been developed. The assay is based on the detection of the lipoxygenase reaction product, linoleic acid hydroperoxide, by the oxidative coupling of 3-methyl-2-benzothiazolinone (MBTH) with 3-(dimethylamino)benzoic acid (DMAB) in a hemoglobin-catalyzed reaction. This test reaction is rapid and sensitive, and it offers advantages over other methods for detecting lipoxygenase activity. The assay is capable of detecting activity in a number of crude vegetable homogenates and should be particularly useful where a rapid visual determination of lipoxygenase activity is desired.

引用

页码：32 / 37

页数：6

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