The prevalence and clonal diversity of penicillin-resistant Streptococcus pneumoniae in Kuwait

被引:11
作者
Ahmed, K
Martinez, G
Wilson, S
Yoshida, R
Dhar, R
Mokaddas, E
Kohno, S
Rotimi, VO
Nagatake, T
机构
[1] Nagasaki Univ, Inst Trop Med, Dept Internal Med, Nagasaki 8528102, Japan
[2] Kuwait Univ, Fac Med, Dept Microbiol, Kuwait, Kuwait
[3] Nagasaki Univ Hosp, Dept Internal Med 2, Nagasaki, Japan
[4] Adan Hosp, Dept Microbiol, Kuwait, Kuwait
关键词
D O I
10.1017/S0950268800004751
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Penicillin-resistant Streptococcus pneumoniae (PRSP) is widespread all over the world, including countries previously free of PRSP. This study was undertaken to determine the prevalence, the common serotypes and the clonality of PRSP isolated over a period of I year, from various clinical samples from three major hospitals in Kuwait. Strains were identified by standard methods and their antibiotic susceptibility was determined by the agar dilution method. The clonality of the isolates was determined by repetitive extragenic palindromic sequence-polymerase chain reaction (REP-PCR) genomic profiling and pulsed held gel electrophoresis (PFGE). Serotyping was done by Quellung reaction using specific antisera. We found that 55% of the S. pneumoniae were resistant to penicillin (46% and 9% exhibited intermediate and full resistance, respectively). Nearly 41% were resistant to sulfamethoxazole-trimethoprim, 9% to cefotaxime and ceftriaxone, 15% to amoxycillin-clavulanate, 17% to cefuroxime, 77% to cefaclor, and 14% to clindamycin. The commonest serotypes among the PRSPs were 6A, 6B, 14, 19F, 23F and nontypable. PFGE and REP-PCR patterns showed a large diversity of genetic clones of the PRSP. Serotypes 6B, 14, 19F and 23F were more clonally related than the others. Our data showed that the prevalence of PRSP was high, the serotypes were diversified and different genetic clones make up the population of circulating PRSP in Kuwait.
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页码:573 / 581
页数:9
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