Axonal surface molecules act in combination with Semaphorin 3A during the establishment of corticothalamic projections

被引:50
作者
Bagnard, D
Chounlamountri, N
Püschel, AW
Bolz, J
机构
[1] INSERM, U371, F-69500 Bron, France
[2] Max Planck Inst Hirnforsch, Neurochem Abt, D-60528 Frankfurt, Germany
[3] Univ Jena, Inst Allgemeine Zool, D-07743 Jena, Germany
关键词
D O I
10.1093/cercor/11.3.278
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Interactions between growing axons are considered to play important roles for the establishment of precise neuronal connections during the development of the nervous system, Here we used time-lapse imaging techniques to examine the behavior of neocortical and thalamic axons when they encounter each other in vitro. Results indicate that axonal growth cones are able to respond to specific cues expressed on the surface of fibers. Thalamic growth cones often extended along the surface of other thalamic axons and, likewise, cortical growth cones formed fascicles with cortical axons, In contrast, after contacts between cortical and thalamic fibers, in most cases growth cones collapsed and retracted from the axons. Collapse assays using membrane preparations from cortical or thalamic explants demonstrated the existence of cell-type specific collapsing factors whose activity was enhanced by a member of the semaphorin protein family, Sema3A (expressed in the thalamocortical pathway), as it increased the rate of homotypic fasciculations and at the same time amplified the segregation between cortical and thalamic axons. The interaction between axonal surface molecules and environmental cues might mediate the segregation of afferent and efferent fiber tracts in the neocortical white matter.
引用
收藏
页码:278 / 285
页数:8
相关论文
共 51 条
[11]   SPATIAL REGULATION OF AXONAL GLYCOPROTEIN EXPRESSION ON SUBSETS OF EMBRYONIC SPINAL NEURONS [J].
DODD, J ;
MORTON, SB ;
KARAGOGEOS, D ;
YAMAMOTO, M ;
JESSELL, TM .
NEURON, 1988, 1 (02) :105-116
[12]   IN-VITRO GUIDANCE OF RETINAL GANGLION-CELL AXONS BY RAGS, A 25 KDA TECTAL PROTEIN RELATED TO LIGANDS FOR EPH RECEPTOR TYROSINE KINASES [J].
DRESCHER, U ;
KREMOSER, C ;
HANDWERKER, C ;
LOSCHINGER, J ;
NODA, M ;
BONHOEFFER, F .
CELL, 1995, 82 (03) :359-370
[13]  
GHOSH A, 1992, J NEUROSCI, V12, P39
[14]  
GOTZ M, 1992, DEVELOPMENT, V116, P507
[15]  
HenkeFahle S, 1996, J NEUROSCI, V16, P4195
[16]   Growth-cone attraction to netrin-1 is converted to repulsion by laminin-1 [J].
Höpker, VH ;
Shewan, D ;
Tessier-Lavigne, M ;
Poo, MM ;
Holt, C .
NATURE, 1999, 401 (6748) :69-73
[17]   GUIDANCE OF THALAMOCORTICAL AXONS BY GROWTH-PROMOTING MOLECULES IN DEVELOPING RAT CEREBRAL-CORTEX [J].
HUBENER, M ;
GOTZ, M ;
KLOSTERMANN, S ;
BOLZ, J .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1995, 7 (09) :1963-1972
[18]  
KAPFHAMMER JP, 1987, J NEUROSCI, V7, P1595
[19]  
KAPFHAMMER JP, 1987, J NEUROSCI, V7, P201
[20]   NETRINS ARE DIFFUSIBLE CHEMOTROPIC FACTORS FOR COMMISSURAL AXONS IN THE EMBRYONIC SPINAL-CORD [J].
KENNEDY, TE ;
SERAFINI, T ;
DELATORRE, JR ;
TESSIERLAVIGNE, M .
CELL, 1994, 78 (03) :425-435