DNA monolayer on gold substrates characterized by nanoparticle labeling and scanning force microscopy

被引：91

作者：

Csáki, A ^{[1
]}

Möller, R ^{[1
]}

Straube, W ^{[1
]}

Köhler, JM ^{[1
]}

Fritzsche, W ^{[1
]}

机构：

[1] Inst Phys High Technol, D-07702 Jena, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2001年 / 29卷 / 16期

关键词：

D O I：

10.1093/nar/29.16.e81

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monolayers of single-stranded DNA on gold substrates were studied by scanning force microscopy. Complementary DNA probes labeled by gold nanoparticles were applied for contrast enhancement. Substrate regions modified with DNA could be visualized in a highly specific manner. The influence of the solution concentration on the surface density of adsorbed nanoparticles could be visualized. Because individual label particles can be easily detected, this labeling technique opens the way for characterization of DNA monolayers with a lateral resolution in the nanometer range.

引用

页码：art. no. / e81

页数：5

共 23 条

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