The human Burkitt lymphoma cell line Namalwa represents a homogenous cell system characterized by high levels of Toll-like receptor 9 and activation by CpG oligonucleotides

被引：22

作者：

Henault, M ^{[1
]}

Lee, LN ^{[1
]}

Evans, GF ^{[1
]}

Zuckerman, SH ^{[1
]}

机构：

[1] Eli Lilly & Co, Lilly Res Labs, Div Bone & Inflammat Res, Indianapolis, IN 46285 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2005年 / 300卷 / 1-2期

关键词：

Toll-like receptors; Burkitt lymphoma; costimulator molecules; cytokines;

D O I：

10.1016/j.jim.2005.02.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human B cells and plasmacytoid dendritic cells constitutively express Toll-like receptor (TLR)9 and respond to TLR9 ligands, as evidenced by nuclear factor kappa B translocation and cytokine secretion. However, TLR9 expression on B lymphocytes appears to be dependent upon both the state of activation and differentiation of the B cell population. In the current study, TLR9 mRNA expression was evaluated in transformed human B cell lines and correlated with their response to CpG. Among the B cell lines, the Burkitt lymphoma-derived Namalwa line had the highest level of TLR9 expression, 20-fold greater than spleen. Following incubation with CpG oligonucleotide, Namalwa cells secreted increased amounts of TNF, IL-6, and IL10 and expressed the costimulator molecules CD40, CD80, and CD86. These functional responses to TLR9 activation occurred with similar EC50 values in the 30- to 60-nM range. These results suggest that the Namalwa Burkitt lymphoma line may serve as a useful cell-based assay for the detection of novel TLR9 agonists as well as a model to further explore the regulation of TLR9 expression and signaling. (c) 2005 Elsevier B.V All rights reserved.

引用

页码：93 / 99

页数：7

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