Analysis of Helicobacter pylori isolates from Chile:: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide

被引：15

作者：

Altman, Eleonora ^{[1
]}

Fernandez, Heriberto ^{[2
]}

Chandan, Vandana ^{[1
]}

Harrison, Blair A. ^{[1
]}

Schuster, Myra Wilson ^{[2
]}

Rademacher, Laura Otth ^{[2
]}

Toledo, Claudio ^{[3
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

[2] Univ Austral Chile, Fac Med, Inst Clin Microbiol, Valdivia, Chile

[3] Univ Austral Chile, Fac Med, Inst Med, Valdivia, Chile

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2008年 / 57卷 / 05期

关键词：

D O I：

10.1099/jmm.0.47783-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84%), whilst 9/38 isolates (24%) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16%) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3%).

引用

页码：585 / 591

页数：7

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