Searching for tagged male-sterile mutants of arabidopsis

被引:11
作者
Glover, JA
Blomer, KC
Farrell, LB
Chaudhury, AM
Dennis, ES
机构
[1] CSIRO,DIV PLANT IND,CANBERRA,ACT 2601,AUSTRALIA
[2] COOPERAT RES CTR PLANT SCI,CANBERRA,ACT 2601,AUSTRALIA
[3] AUSTRALIAN NATL UNIV,DIV BIOCHEM & MOL BIOL,CANBERRA,ACT 0200,AUSTRALIA
关键词
Arabidopsis; male-sterile mutant; T-DNA;
D O I
10.1007/BF02673365
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Although many male-sterile mutants have been identified in Arabidopsis thaliana, few of the corresponding genes have been cloned. In order to facilitate cloning of a male sterility gene, 23 of Feldmann's T-DNA-generated, reduced-fertility lines were screened to identify a tagged male-sterile mutation. Male-sterile mutants were identified, as well as mutants that were both male and female sterile. Segregation of the kanamycin marker gene in the progeny of 15 of these lines was studied. Forty percent had functional T-DNAs (encoding resistance to kanamycin) inserted at a single locus, the remainder segregating for two or more functional T-DNA inserts. Linkage between T-DNA inserts and mutant phenotype was tested for six lines. In three of these lines, mutations were not linked to a T-DNA insert. In three lines, the mutation segregated with a T-DNA insert.
引用
收藏
页码:330 / 342
页数:13
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