Isolation of human intestinal defensins from ileal neobladder urine

被引：64

作者：

Porter, EM

Poles, MA

Lee, JS

Naitoh, J

Bevins, CL

Ganz, T ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Will Rogers Inst Pulm Res, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Div Digest Dis, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Dept Urol, Los Angeles, CA 90095 USA

[5] Cleveland Clin Fdn, Dept Immunol Gastroenterol & Colorectal Surg, Cleveland, OH 44195 USA

来源：

FEBS LETTERS | 1998年 / 434卷 / 03期

关键词：

small intestine; mucosal immunity; antimicrobial peptide; cryptdin; phospholipase A2; ileal neobladder;

D O I：

10.1016/S0014-5793(98)00994-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa, Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than these in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation, Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion. (C) 1998 Federation of European Biochemical Societies.

引用

页码：272 / 276

页数：5

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