Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, chicken and turkey

被引:76
作者
Koeppel, Rene [1 ]
Ruf, Jueg [2 ]
Zimmerli, Franziska [1 ]
Breitenmoser, Alda [1 ]
机构
[1] Offcial Food Control Author Canton Zurich, Zurich, Switzerland
[2] Offcial Food Control Author Canton Thurgau, Zurich, Switzerland
关键词
multiplex; real-time quantitative PCR; beef; pork; chicken; turkey;
D O I
10.1007/s00217-008-0837-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Many meat products are composed of two or more meat species. To determine the proportion of these meat fractions, a quantitative multiplex PCR was developed for the quantification of beef, pork, chicken and turkey. This system proved its applicability, precision and accuracy in examining different meat products from the market. Thus it allows the efficient control of composed meat products in official food control and production control laboratories.
引用
收藏
页码:1199 / 1203
页数:5
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