In vitro synthesis of artificial polysaccharides by glycosidases and glycosynthases

被引:81
作者
Faijes, Magda [1 ]
Planas, Antoni [1 ]
机构
[1] Univ Ramon Llull, Inst Quim Sarria, Biochem Lab, Barcelona 08017, Spain
关键词
glycosidases; glycosynthases; polysaccharides; enzymatic synthesis; biomaterials;
D O I
10.1016/j.carres.2007.06.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Artificial polysaccharides produced by in vitro enzymatic synthesis are new biomaterials with defined structures that either mimic natural polysaccharides or have unnatural structures and functionalities. This review summarizes recent developments in the in vitro polysaccharide synthesis by endo-glycosidases, grouped in two major strategies: (a) native retaining endo-glycosidases under kinetically controlled conditions (transglycosylation with activated glycosyl donors), and (b) glycosynthases, engineered glycosidases devoid of hvdrolase activity but with high transglycosylation activity. Polysaccharides, are obtained by enzymatic polymerization of simple glycosyl donors by repetitive condensation. This approach not only provides a powerful methodology to produce polysaccharides with defined structures and morphologies as novel biomaterials, but is also a valuable tool to analyze the mechanisms of polymerization and packing to acquire high-order molecular assemblies. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1581 / 1594
页数:14
相关论文
共 117 条
[1]   Review. Hyaluronan: A powerful tissue engineering tool [J].
Allison, David D. ;
Grande-Allen, K. Jane .
TISSUE ENGINEERING, 2006, 12 (08) :2131-2140
[2]   A bifunctionalized fluorogenic tetrasaccharide as a substrate to study cellulases [J].
Armand, S ;
Drouillard, S ;
Schulein, M ;
Henrissat, B ;
Driguez, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (05) :2709-2713
[3]  
BENDAVID A, 2007, 7 CARB BIOENG M BRAU
[4]   Chemoenzymatic synthesis of glycan libraries [J].
Blixt, Ola ;
Razi, Nahid .
GLYCOBIOLOGY, 2006, 415 :137-+
[5]  
Boyer V, 2002, CHEM-EUR J, V8, P1389, DOI 10.1002/1521-3765(20020315)8:6<1389::AID-CHEM1389>3.0.CO
[6]  
2-#
[7]   Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus [J].
Cheng, Chao-Chun ;
Yu, Mei-Ching ;
Cheng, Tzu-Chien ;
Sheu, Dey-Chyi ;
Duan, Kow-Jen ;
Tai, Wei-Lun .
BIOTECHNOLOGY LETTERS, 2006, 28 (11) :793-797
[8]   Synthesis of galacto-oligosaccha ride from lactose using β-galactosidase from Kluyveromyces lactis:: Studies on batch and continuous UF membrane-fitted bioreactors [J].
Chockchaisawasdee, S ;
Athanasopoulos, VI ;
Niranjan, K ;
Rastall, RA .
BIOTECHNOLOGY AND BIOENGINEERING, 2005, 89 (04) :434-443
[9]  
Davies G., 1998, COMPREHENSIVE BIOL C, P119
[10]   Recent developments in oligosaccharide synthesis [J].
Davis, BG .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 2000, (14) :2137-2160