Purification and characterization of adenosine diphosphate glucose pyrophosphorylase from maize/potato mosaics

被引:29
作者
Boehlein, SK
Sewell, AK
Cross, J
Stewart, JD
Hannah, LC [1 ]
机构
[1] Univ Florida, Program Plant Mol & Cellular Biol & Hort Sci, Gainesville, FL 32611 USA
[2] Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32611 USA
[3] Univ Florida, Dept Chem, Gainesville, FL 32611 USA
关键词
D O I
10.1104/pp.105.060699
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Adenosine diphosphate glucose pyrophosphorylase (AGPase) catalyzes a rate-limiting step in starch biosynthesis. The reaction produces ADP-glucose and pyrophosphate from glucose-1-P and ATP. Investigations from a number of laboratories have shown that alterations in allosteric properties as well as heat stability of this enzyme have dramatic positive effects on starch synthesis in the potato (Solanum tuberosum) tuber and seeds of important cereals. Here, we report the characterization of purified recombinant mosaic AGPases derived from protein motifs normally expressed in the maize (Zea mays) endosperm and the potato tuber. These exhibit properties that should be advantageous when expressed in plants. We also present an in- depth characterization of the kinetic and allosteric properties of these purified recombinant AGPases. These data point to previously unrecognized roles for known allosteric effectors.
引用
收藏
页码:1552 / 1562
页数:11
相关论文
共 35 条
[31]   PYROPHOSPHORYLASES IN SOLANUM-TUBEROSUM .3. PURIFICATION, PHYSICAL, AND CATALYTIC PROPERTIES OF ADP-GLUCOSE PYROPHOSPHORYLASE IN POTATOES [J].
SOWOKINOS, JR ;
PREISS, J .
PLANT PHYSIOLOGY, 1982, 69 (06) :1459-1466
[32]   PYROPHOSPHORYLASES IN SOLANUM-TUBEROSUM .2. CATALYTIC PROPERTIES AND REGULATION OF ADP-GLUCOSE AND UDP-GLUCOSE PYROPHOSPHORYLASE ACTIVITIES IN POTATOES [J].
SOWOKINOS, JR .
PLANT PHYSIOLOGY, 1981, 68 (04) :924-929
[33]   REGULATION OF THE AMOUNT OF STARCH IN PLANT-TISSUES BY ADP GLUCOSE PYROPHOSPHORYLASE [J].
STARK, DM ;
TIMMERMAN, KP ;
BARRY, GF ;
PREISS, J ;
KISHORE, GM .
SCIENCE, 1992, 258 (5080) :287-292
[34]   Starch synthesis in potato tubers is regulated by post-translational redox modification of ADP-glucose pyrophosphorylase:: A novel regulatory mechanism linking starch synthesis to the sucrose supply [J].
Tiessen, A ;
Hendriks, JHM ;
Stitt, M ;
Branscheid, A ;
Gibon, Y ;
Farré, EM ;
Geigenberger, P .
PLANT CELL, 2002, 14 (09) :2191-2213
[35]   Kinetic and crystallographic analyses support a sequential-ordered BiBi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferase [J].
Zuccotti, S ;
Zanardi, D ;
Rosano, C ;
Sturla, L ;
Tonetti, M ;
Bolognesi, M .
JOURNAL OF MOLECULAR BIOLOGY, 2001, 313 (04) :831-843