High efficiency of transferring a native sugar chain from a glycopeptide by a microbial endoglycosidase in organic solvents

被引：30

作者：

Akaike, E

Tsutsumida, M

Osumi, K

Fujita, M

Yamanoi, T

Yamamoto, K

Fujita, K

机构：

[1] Noguchi Inst, Itabashi Ku, Tokyo 1730003, Japan

[2] Kyoto Univ, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068502, Japan

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 03期

关键词：

endo-beta-N-acetylglucosaminidase; Endo-M; transglycosylation; organic solvent; biantennary complex-type oligosaccharide; neoglycoconjugates;

D O I：

10.1016/j.carres.2003.12.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the transglycosylation reaction by the recombinant endo-beta-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) expressed in Candida boidinii in media containing organic solvents. The recombinant Endo-M could transglycosylate a disialo biantennary complex-type oligosaccharide from hen egg yolk glycopeptide to p-nitrophenyl N-acetyl-beta-D-glucosaminide even in the presence of 30% acetone, dimethyl sulfoxide, or methanol. The yield of the transglycosylation product reached 21-34% of the total amount of acceptor, while the yield was only about 14% in aqueous solution. (C) 2003 Elsevier Ltd. All rights reserved.

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页码：719 / 722

页数：4

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