Construction of a BAC library of pearl millet, Pennisetum glaucum

被引：77

作者：

Allouis, S ^{[1
]}

Qi, X ^{[1
]}

Lindup, S ^{[1
]}

Gale, MD ^{[1
]}

Devos, KM ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

来源：

THEORETICAL AND APPLIED GENETICS | 2001年 / 102卷 / 08期

关键词：

bacterial artificial chromosome (BAC); pearl millet; Pennisetum;

D O I：

10.1007/s001220100559

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A bacterial artificial chromosome (BAC) library was constructed using nuclear DNA from pearl miller (Pennisetum glaucum), and used as a resource for the isolation of microsatellite sequences. The library contains a total of 159,100 clones with an average insert size of 90 kb, and corresponds to 5.8 haploid genome equivalents. The BAC library was pooled for screening by the polymerase chain reaction (PCR) as well as robotically gridded on high-density filters. PCR-based screening of a subset of the library (4.7 haploid genome equivalents) using five sequence-tagged site (STS) and six microsatellite markers identified between 2 and 11 positives superpools (5.4 on average). The frequency of BAC clones carrying inserts of chloroplast DNA was estimated to be less than 1% by hybridisation with a rice chloroplast probe.

引用

页码：1200 / 1205

页数：6

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