Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis

被引:169
作者
Akineden, Ö
Annemüller, C
Hassan, AA
Lämmler, C
Wolter, W
Zschöck, M
机构
[1] Univ Giessen, Inst Tierarztliche Nahrungsmittelkunde, D-35392 Giessen, Germany
[2] Staatliches Med Lebensmittel & Vet Untersuchungsa, D-35396 Giessen, Germany
关键词
D O I
10.1128/CDLI.8.5.959-964.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In the present study, 103 Staphylococcus aureus strains isolated from milk samples from 60 cows with mastitis from eight different farms in seven different locations in one region of Germany were compared pheno- and genotypically and by identification of various toxins. On the basis of culture and hemolytic properties and by determination of the tube coagulase reaction, all of the isolates could be identified as S. aureus. This could be confirmed by PCR amplification of species-specific parts of the gene encoding the 23S rRNA. In addition, all of the S. aureus isolates harbored the genes encoding staphylococcal coagulase and clumping factor and the genes encoding the X region and the immunoglobulin G binding region of protein A. These four genes displayed size polymorphisms. By PCR amplification, the genes for the toxins staphylococcal enterotoxin A (SEA), SEC, SED, SEG, SEI, SEJ, and TSST-1 but not those for SEB, SEE, SEH, and the exfoliative toxins ETA and ETB could be detected. To analyze the epidemiological relationships, the isolates were subjected to DNA fingerprinting by macrorestriction analysis of their chromosomal DNAs. According to the observed gene polymorphisms, the toxin patterns, and the information given by macrorestriction analysis of the isolates by pulsed-field gel electrophoresis, a limited number of clones seemed to be responsible for the cases of bovine mastitis on the various farms.
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页码:959 / 964
页数:6
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共 51 条
  • [11] Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows
    Fitzgerald, JR
    Meaney, WJ
    Hartigan, PJ
    Smyth, CJ
    Kapur, V
    [J]. EPIDEMIOLOGY AND INFECTION, 1997, 119 (02) : 261 - 269
  • [12] Molecular population and virulence factor analysis of Staphylococcus aureus from bovine intramammary infection
    Fitzgerald, JR
    Hartigan, PJ
    Meaney, WJ
    Smyth, CJ
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (06) : 1028 - 1037
  • [13] Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions
    Forsman, P
    TilsalaTimisjarvi, A
    Alatossava, T
    [J]. MICROBIOLOGY-UK, 1997, 143 : 3491 - 3500
  • [14] Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism
    Frenay, HME
    Bunschoten, AE
    Schouls, LM
    vanLeeuwen, WJ
    VandenbrouckeGrauls, CMJE
    Verhoef, J
    Mooi, FR
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (01) : 60 - 64
  • [15] FRENAY HME, 1994, J CLIN MICROBIOL, V32, P846
  • [16] MOLECULAR TYPING OF STAPHYLOCOCCUS-AUREUS ON THE BASIS OF COAGULASE GENE POLYMORPHISMS
    GOH, SH
    BYRNE, SK
    ZHANG, JL
    CHOW, AW
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) : 1642 - 1645
  • [17] Antibody response to toxic shock syndrome toxin-1 of Staphylococcus aureus in dairy cows
    Hayakawa, Y
    Akagi, M
    Hayashi, M
    Shimano, T
    Komae, H
    Funaki, O
    Kaidoh, T
    Takeuchi, S
    [J]. VETERINARY MICROBIOLOGY, 2000, 72 (3-4) : 321 - 327
  • [18] Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene
    Hookey, JV
    Richardson, JF
    Cookson, BD
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) : 1083 - 1089
  • [19] Involvement of enterotoxins G and I in staphylococcal toxic shock syndrome and staphylococcal scarlet fever
    Jarraud, S
    Cozon, G
    Vandenesch, F
    Bes, M
    Etienne, J
    Lina, G
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (08) : 2446 - 2449
  • [20] DETECTION OF GENES FOR ENTEROTOXINS, EXFOLIATIVE TOXINS, AND TOXIC SHOCK SYNDROME TOXIN-1 IN STAPHYLOCOCCUS-AUREUS BY THE POLYMERASE CHAIN-REACTION
    JOHNSON, WM
    TYLER, SD
    EWAN, EP
    ASHTON, FE
    POLLARD, DR
    ROZEE, KR
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (03) : 426 - 430