Sensitive Detection of Spores Using Volume-Amplified Magnetic Nanobeads

被引：28

作者：

de la Torre, Teresa Zardan Gomez ^{[1
]}

Ke, Rongqin ^{[2
]}

Mezger, Anja ^{[2
]}

Svedlindh, Peter ^{[3
]}

Stromme, Maria ^{[1
]}

Nilsson, Mats ^{[2
]}

机构：

[1] Uppsala Univ, Angstrom Lab, Div Nanotechnol & Funct Mat, Dept Engn Sci, S-75121 Uppsala, Sweden

[2] Uppsala Univ, Rudbeck Lab, Dept Immunol Genet & Pathol Sci Life Lab, S-75185 Uppsala, Sweden

[3] Uppsala Univ, Angstrom Lab, Div Solid State Phys, Dept Engn Sci, S-75121 Uppsala, Sweden

来源：

SMALL | 2012年 / 8卷 / 14期

基金：

瑞典研究理事会;

关键词：

biosensors; immunoassays; magnetic particles; spores; nanobeads; PROXIMITY LIGATION ASSAYS; DNA-COILS; NANOPARTICLES; BIOSENSORS; ATTACHMENT;

D O I：

10.1002/smll.201102632

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A magnetic-nanobead-based, substrate-free method for the sensitive detection of spores in an immunoassay format is presented. The method is shown to detect Bacillus globigii spores, the non-pathogenic simulant of Bacillus anthracis, with a limit-of-detection of 50 spores with a reaction time of 135 min. The study shows the versatility of magnetic nanobeads for detection of biological molecules other than DNA. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2174 / 2177

页数：4

共 23 条

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