Detection of rolling circle amplified DNA molecules using probe-tagged magnetic nanobeads in a portable AC susceptometer

被引：55

作者：

de la Torre, Teresa Zardan Gomez ^{[1
]}

Mezger, Anja ^{[2
]}

Herthnek, David

Johansson, Christer ^{[3
]}

Svedlindh, Peter ^{[4
]}

Nilsson, Mats

Stromme, Maria ^{[1
]}

机构：

[1] Uppsala Univ, Div Nanotechnol & Funct Mat, Dept Engn Sci, Angstrom Lab, SE-75121 Uppsala, Sweden

[2] Uppsala Univ, Dept Immunol Genet & Pathol, Sci Life Lab, Rudbeck Lab, SE-75185 Uppsala, Sweden

[3] Chalmers Ind, SE-41288 Gothenburg, Sweden

[4] Uppsala Univ, Div Solid State Phys, Dept Engn Sci, Angstrom Lab, SE-75121 Uppsala, Sweden

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 29卷 / 01期

基金：

瑞典研究理事会;

关键词：

Probe-tagged magnetic beads; Padlock probes; Rolling circle amplification; Brownian relaxation; AC susceptometer; SENSITIVE DETECTION; DETECTION ASSAY; PADLOCK PROBES; IN-SITU; NANOPARTICLES; BIOSENSORS; SEQUENCES; SENSOR; ARRAY; OLIGONUCLEOTIDES;

D O I：

10.1016/j.bios.2011.08.019

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Here, the volume-amplified magnetic nanobead detection assay (VAM-NDA) is for the first time applied for detection of rolling circle amplified (RCA) DNA molecules in a portable, commercial AC susceptometer that operates at ambient temperatures and with an analysis time of about 20 min. The performance of the assay is investigated using three different magnetic nanobead sizes: 50, 130 and 250 nm. The performance of the assay using the AC susceptometer is compared to the performance achieved using a superconducting quantum interference device (SQUID). It is found that the performance of the assay is comparable in the two setups with a quantitative detection limit of similar to 4 pM for all bead sizes under study. The findings show that the VAM-NDA holds promise for future wide-spread implementation in commercial AC susceptometer setups thus opening up for the possibility to perform magnetic bead-based DNA detection in point-of-care and outpatient settings. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：195 / 199

页数：5

共 37 条

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