Isolation and characterization of proteoglycans from growing antlers of wapiti (Cervus elaphus)

被引：28

作者：

Sunwoo, HH ^{[1
]}

Nakano, T ^{[1
]}

Sim, JS ^{[1
]}

机构：

[1] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1998年 / 121卷 / 04期

关键词：

antlers; chondrocytes; chondroitin sulfate; decorin; keratan sulfate; proteoglycans; wapiti;

D O I：

10.1016/S0305-0491(98)10127-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteoglycans were extracted with 4 M guanidine-HCl from the zone of maturing chondrocytes, the site of endochondral ossification of growing antlers of wapiti (Cervus elaphus), Proteoglycans were isolated by DEAE-Sephacel chromatography and separated by Sepharose CL-4B chromatography into three fractions. Fraction I contained a high molecular mass (>1000 kDa) chondroitin sulfate proteoglycan capable of interacting with hyaluronic acid. Its amino acid composition resembled that of the cartilage proteoglycan. aggrecan. Fraction II contained proteoglycans with intermediate molecular weight which were recognized by monoclonal antibodies specific to chondroitin sulfate and keratan sulfate. Fraction III contained a low molecular mass (<160 kDa) proteoglycan, decorin, with a glucuronate-rich glycosaminoglycan chain. (C) 1998 Elsevier Science Inc. All rights reserved.

引用

页码：437 / 442

页数：6

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