Chondroltin O-methyl ester:: an unusual substrate for chondroitin AC lyase

被引：6

作者：

Avci, FY

Toida, T

Linhardt, RJ ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Dept Chem, Troy, NY 12180 USA

[2] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Bioanalyt Chem, Chiba 2638522, Japan

来源：

CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 20期

关键词：

chondroitin O-methyl ester; chondroitin AC lyase; Flavobacterium heparinum;

D O I：

10.1016/S0008-6215(03)00348-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Chondroitin O-methyl ester was depolymerized by chondroitin AC lyase (EC 4.2.2.5) from Flavobacterium heparinum. The major product isolated from the depolymerization reaction was found to be methyl alpha-L-threo-hex-4-enopyranosyluronate-(1-->4)-2-acetamido-2-deoxy-alpha,beta-D-galactopyranoside. (C) 2003 Elsevier Ltd. All rights reserved.

引用

收藏

页码：2101 / 2104

页数：4

相关论文

共 15 条

[1]

Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum [J].

Capila, I ;

Wu, Y ;

Rethwisch, DW ;

Matte, A ;

Cygler, M ;

Linhardt, RJ .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1597 (02) :260-270

[2]

ALGINATE-MODIFYING ENZYMES - A PROPOSED UNIFIED MECHANISM OF ACTION FOR THE LYASES AND EPIMERASES [J].

GACESA, P .

FEBS LETTERS, 1987, 212 (02) :199-202

[3]

UNDERSTANDING ENZYME-CATALYZED PROTON ABSTRACTION FROM CARBON ACIDS - DETAILS OF STEPWISE MECHANISMS FOR BETA-ELIMINATION REACTIONS [J].

GERLT, JA ;

GASSMAN, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (15) :5928-5934

[4]

PURIFICATION, CHARACTERIZATION AND SPECIFICITY OF CHONDROITIN LYASES AND GLYCURONIDASE FROM FLAVOBACTERIUM-HEPARINUM [J].

GU, KN ;

LINHARDT, RJ ;

LALIBERTE, M ;

GU, KF ;

ZIMMERMANN, J .

BIOCHEMICAL JOURNAL, 1995, 312 :569-577

[5]

COMPARISON OF THE ACTIVITY OF 2 CHONDROITIN AC LYASES ON DERMATAN SULFATE [J].

GU, KN ;

LIU, J ;

PERVIN, A ;

LINDHARDT, RJ .

CARBOHYDRATE RESEARCH, 1993, 244 (02) :369-377

[6]

ELECTROSTATIC STABILIZATION CAN EXPLAIN THE UNEXPECTED ACIDITY OF CARBON ACIDS IN ENZYME-CATALYZED REACTIONS [J].

GUTHRIE, JP ;

KLUGER, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (24) :11569-11572

[7]

Hernaiz M J, 2001, Methods Mol Biol, V171, P363

[8]

ACTION OF CHONDROITINASES .1. MODE OF ACTION OF 2 CHONDROITINASE-AC PREPARATIONS OF DIFFERENT ORIGIN [J].

HIYAMA, K ;

OKADA, S .

JOURNAL OF BIOCHEMISTRY, 1976, 80 (06) :1201-1207

[9]

Structure-function relationships of β-D-glucan endo- and exohydrolases from higher plants [J].

Hrmova, M ;

Fincher, GB .

PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :73-91

[10]

Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis [J].

Huang, WJ ;

Boju, L ;

Tkalec, L ;

Su, HS ;

Yang, HO ;

Gunay, NS ;

Linhardt, RJ ;

Kim, YS ;

Matte, A ;

Cygler, M .

BIOCHEMISTRY, 2001, 40 (08) :2359-2372