Induction of beta-methylcrotonyl-coenzyme A carboxylase in higher plant cells during carbohydrate starvation: Evidence for a role of MCCase in leucine catabolism

被引:37
作者
Aubert, S
Alban, C
Bligny, R
Douce, R
机构
[1] CEA GRENOBLE,DBMS,PHYSIOL CELLULAIRE VEGETALE LAB,CNRS,URA 576,F-38054 GRENOBLE 8,FRANCE
[2] RHONE POULENC AGROCHIM,CNRS,LAB MIXTE,UMR 41,F-69263 LYON,FRANCE
关键词
beta-methylcrotonyl-CoA carboxylase; leucine catabolism; plant mitochondria; carbon starvation; autophagy; C-13]NMR; Acer pseudoplatanus;
D O I
10.1016/0014-5793(96)00244-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Induction of beta-methylcrotonyl-coenzyme A carboxylase (MCCase) activity was observed during carbohydrate starvation in sycamore cells. In mitochondria isolated from starved cells, we noticed a marked accumulation of the biotinylated subunit of MCCase, of which the apparent molecular weight of 74 000 was similar to that of the polypeptide from mitochondria of potato tubers. Our results provide evidence for a role of MCCase in the catabolic pathway of leucine, a branched-chain amino acid which transiently accumulates in carbon-starved cells in relation to a massive breakdown of proteins. Furthermore, when control sycamore cells mere incubated in the presence of exogenous leucine, this amino acid accumulated in the cells and no induction or accumulation of MCCase was observed, indicating that leucine is not responsible for the induction of its catabolic machinery. Finally, MCCase is proposed as a new biochemical marker of the autophagic process triggered by carbohydrate starvation.
引用
收藏
页码:175 / 180
页数:6
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