Enhanced protein glutathiolation and oxidative stress in cigarette smokers

被引:67
作者
Muscat, JE
Kleinman, W
Colosimo, S
Muir, A
Lazarus, P
Park, J
Richie, JP
机构
[1] Inst Canc Prevent, Amer Hlth Fdn, Ctr Canc, Valhalla, NY 10595 USA
[2] Univ Florida, H Lee Moffitt Canc Ctr, Tampa, FL USA
关键词
glutathione; glutathiolation; smoking; oxidative stress; genotype; free radicals;
D O I
10.1016/j.freeradbiomed.2003.11.018
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There are many functional assays of oxidative damage to DNA, protein, and lipids but few reliable markers of chronic oxidative stress. The glutathiolation of proteins at key Cys residues is considered an important redox-sensitive, posttranslational signaling mechanism in the regulation of critical cellular functions. To determine whether protein bound glutathione (GSSP) is a sensitive indicator of oxidative stress, red blood cell and plasma concentrations were measured and compared between smokers and nonsmokers. In a community-based study conducted in Westchester County, New York, USA, blood samples were obtained from 354 cigarette smokers and 97 never smokers. The mean concentration of blood GSSP (mumol/L) was 32% higher in cigarette smokers and 43% higher when standardized by hemoglobin concentrations (p < .01). Plasma GSSP levels were also 20% higher in smokers than in nonsmokers (p < .001). The relationship was dose-dependent, with blood GSSP levels significantly correlated with cigarettes smoked per day, plasma cotinine, and plasma thiocyanate (r values ranged from .25 to. 40). In smokers, there were no significant differences in GSSP and GSH levels by GSTM1 or GSTM3 genotype. Intraindividual variation in blood samples, as determined by taking serial samples over a 2-week period, was low (CV = 12.1%, n = 8). GSSP levels are stable over time but increase in response to the abundant free radicals in cigarette smoke. These findings support the use of GSSP as a sensitive biomarker of oxidative stress. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:464 / 470
页数:7
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