High Proportion of Wrongly Identified Methicillin-Resistant Staphylococcus aureus Carriers by Use of a Rapid Commercial PCR Assay Due to Presence of Staphylococcal Cassette Chromosome Element Lacking the mecA Gene

被引：65

作者：

Blanc, Dominique S. ^{[1
,2
]}

Basset, Patrick ^{[1
,2
]}

Nahimana-Tessemo, Immaculee ^{[1
,2
]}

Jaton, Katia

Greub, Gilbert

Zanetti, Giorgio ^{[1
,2
]}

机构：

[1] CHU Vaudois, Serv Med Prevent Hosp, CH-1011 Lausanne, Switzerland

[2] CHU Vaudois, Inst Microbiol, CH-1011 Lausanne, Switzerland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 02期

关键词：

CLINICAL-SAMPLES; SURVEILLANCE;

D O I：

10.1128/JCM.01988-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During a 9-month period, 217 patients were newly diagnosed as methicillin-resistant Staphylococcus aureus (MRSA) carriers by using a commercial rapid PCR-based test (GeneXpert). However, no MRSA was recovered by culturing the second swab in 61 of these patients. Further analyses showed that 28 (12.9%) of the patients harbored S. aureus isolates with a staphylococcal cassette chromosome element lacking the mecA gene and were thus incorrectly determined to be MRSA carriers.

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页码：722 / 724

页数：3

相关论文

共 16 条

[1] Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus [J].

Basset, P. ;

Senn, L. ;

Prod'hom, G. ;

Bille, J. ;

Francioli, P. ;

Zanetti, G. ;

Blanc, D. S. .

CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (08) :1289-1296

[2] Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus [J].

Donnio, PY ;

Oliveira, DC ;

Faria, NA ;

Wilhelm, N ;

Le Coustumier, A ;

de Lencastre, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :4191-4193

[3] Nine-year surveillance of methicillin-resistant Staphylococcus aureus in a hospital suggests instability of mecA DNA region in an epidemic strain [J].

Donnio, PY ;

Louvet, L ;

Preney, L ;

Nicolas, D ;

Avril, JL ;

Desbordes, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) :1048-1052

[4] Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus [J].

Enright, MC ;

Day, NPJ ;

Davies, CE ;

Peacock, SJ ;

Spratt, BG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) :1008-1015

[5] Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay [J].

Fang, H ;

Hedin, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :2894-2899

[6] Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay [J].

Francois, P ;

Pittet, D ;

Bento, M ;

Pepey, B ;

Vaudaux, P ;

Lew, D ;

Schrenzel, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (01) :254-260

[7] Comparison of ATB Staph, Rapid ATB Staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA [J].

Frebourg, NB ;

Nouet, D ;

Lemée, L ;

Martin, E ;

Lemeland, JF .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) :52-57

[8] New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci [J].

Huletsky, A ;

Giroux, R ;

Rossbach, V ;

Gagnon, M ;

Vaillancourt, M ;

Bernier, M ;

Gagnon, F ;

Truchon, K ;

Bastien, M ;

Picard, FJ ;

van Belkum, A ;

Ouellette, M ;

Roy, PH ;

Bergeron, MG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :1875-1884

[9] Identification of methicillin-resistant Staphylococcus aureus carriage in less than 1 hour during a hospital surveillance program [J].

Huletsky, A ;

Lebel, P ;

Picard, FJ ;

Bernier, M ;

Gagnon, M ;

Boucher, N ;

Bergeron, MG .

CLINICAL INFECTIOUS DISEASES, 2005, 40 (07) :976-981

[10] Insights on antibiotic resistance of Staphylococcus aureus from its whole genome:: genomic island SCC [J].

Ito, T ;

Okuma, K ;

Ma, XX ;

Yuzawa, H ;

Hiramatsu, K .

DRUG RESISTANCE UPDATES, 2003, 6 (01) :41-52