Analysis of constitutive cytokine expression by pigs infected in-utero with porcine reproductive and respiratory syndrome virus

被引:30
作者
Feng, WH
Tompkins, MB
Xu, JS
Zhang, HX
McCaw, MB
机构
[1] N Carolina State Univ, Coll Vet Med, Dept Farm Anim Hlth & Resource Management, Raleigh, NC 27606 USA
[2] N Carolina State Univ, Coll Vet Med, Dept Microbiol Pathol & Parasitol, Raleigh, NC 27606 USA
[3] Beijing Entry Exit Inspect & Quarantine Bur, Quarantine Sect, Beijing 100022, Peoples R China
关键词
porcine reproductive and respiratory syndrome virus; cytokine; delayed type hypersensitivity; peripheral blood mononuclear cells; RT-PCR;
D O I
10.1016/S0165-2427(03)00059-X
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 [免疫学];
摘要
To investigate cytokine alterations in pigs infected in-utero with porcine reproductive and respiratory syndrome virus (PRRSV), constitutive mRNA expression by peripheral blood mononuclear cells (PBMCs) was measured. PBMC from in-utero PRRSV-infected pigs displayed significantly increased IL-6, IL-10, and IFN-gamma mRNA expression at 0 and 14 days of age compared with age-matched control pigs. There were no significant differences in IL-2, IL-4, and IL-12 mRNA expression between in-utero PRRSV-infected and control pigs. However, the IL-10/IL-12 ratio was significantly increased in in-utero PRRSV-infected pigs at 0 and 14 days of age, suggesting the imbalance of IL-10 and IL-12 mRNA production. The abnormal mRNA expression of cytokines in in-utero PRRSV-infected pigs occurred concurrently with a significant decrease in the CD4(+)/CD8(+) T-cell ratio in peripheral blood. PRRSV was not isolated from the sera of pigs at 9 weeks of age that had been viremic at 0 and 14 days old. Delayed type hypersensitivity (DTH) responses to Tuberculin and analysis of cytokine mRNA expression by PBMC showed that cell-mediated immune response and cytokine message profiles in pigs infected in-utero with PRRSV had returned to levels similar to those of control pigs by 9 weeks of age. We conclude that in-utero infection with PRRSV results in significant alteration of cytokine mRNA expression that may cause transient immunomodulation. However, at 10 weeks of age the pigs' immune responses seemed to recover. This may help to understand the immunopathogenesis of in-utero PRRSV infection and the increased susceptibility to secondary bacterial pathogens in neonatal piglets. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:35 / 45
页数:11
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