Drinking a hot blood meal elicits a protective heat shock response in mosquitoes

被引：107

作者：

Benoit, Joshua B. ^{[1
,2
,3
]}

Lopez-Martinez, Giancarlo ^{[1
,2
,4
]}

Patrick, Kevin R. ^{[1
,2
]}

Phillips, Zachary P. ^{[1
,2
]}

Krause, Tyler B. ^{[1
,2
]}

Denlinger, David L. ^{[1
,2
]}

机构：

[1] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA

[2] Ohio State Univ, Dept Ecol Evolut & Organismal Biol, Columbus, OH 43210 USA

[3] Yale Univ, Sch Publ Hlth, Div Epidemiol Microbial Dis, New Haven, CT 06510 USA

[4] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 19期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

AEDES-AEGYPTI DIPTERA; GENE-EXPRESSION; ANOPHELES-GAMBIAE; HSP70; EXPRESSION; STRESS-RESPONSE; DIURESIS; MIDGUT; TRANSCRIPTS; TOLERANCE; PROTEINS;

D O I：

10.1073/pnas.1105195108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mosquito's body temperature increases dramatically when it takes a blood meal from a warm-blooded, vertebrate host. By using the yellow fever mosquito, Aedes aegypti, we demonstrate that this boost in temperature following a blood meal prompts the synthesis of heat shock protein 70 (Hsp70). This response, elicited by the temperature of the blood meal, is most robust in the mosquito's midgut. When RNA interference is used to suppress expression of hsp70, protein digestion of the blood meal is impaired, leading to production of fewer eggs. We propose that Hsp70 protects the mosquito midgut from the temperature stress incurred by drinking a hot blood meal. Similar increases in hsp70 were documented immediately after blood feeding in two other mosquitoes (Culex pipiens and Anopheles gambiae) and the bed bug, Cimex lectularius, suggesting that this is a common protective response in blood-feeding arthropods.

引用

页码：8026 / 8029

页数：4

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