Analysis of two Staphylococcus epidermidis plasmids coding for resistance to streptogramin A

被引：12

作者：

Aubert, S

Dyke, KGH

El Solh, N ^{[1
]}

机构：

[1] Inst Pasteur, Ctr Natl Reference Staphylocoques, Unite Staphylocoques, F-75724 Paris 15, France

[2] Univ Oxford, Dept Biochem, Microbiol Unit, Oxford OX1 3QU, England

来源：

PLASMID | 1998年 / 40卷 / 03期

关键词：

D O I：

10.1006/plas.1998.1369

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The two Staphylococcus epidermidis plasmids pIP1629 (7.5 kb) and pIP1630 (14.4 kb) contain the vga gene conferring resistance to streptogramin A. All the sequences of pIP1629, except two of the four 22-nt iterons preceding the replication gene were found in pIP1630. The additional 6.9-kb fragment of pIP1630 is similar to the mobilizable S. epidermidis plasmid pSK639, carrying the dfrA-thyE-orf140 operon and thought to replicate by an iteron controlled theta-type replication mechanism. The replication-mobilization elements of pIP1629 and pSK639 are very similar despite having been isolated in France and in Australia, respectively, showing that they are geographically widely dispersed in S. epidermidis. The gene thyE encoding thymidylate synthetase carried by pSK639 is not present in pIP1630. pIP1630 probably arose by the recombination of two homologous plasmids carrying distinct resistance determinants. (C) 1998 Academic Press.

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页码：238 / 242

页数：5

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