A rapid DNA extraction method for PCR identification of fungal indoor air contaminants

被引：24

作者：

Dean, TR ^{[1
]}

Betancourt, D ^{[1
]}

Menetrez, MY ^{[1
]}

机构：

[1] US EPA, Natl Risk Management Res Lab, Res Triangle Pk, NC 27711 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 56卷 / 03期

关键词：

PCR inhibitors; DNA extraction; Stachybotrys chartarum;

D O I：

10.1016/j.mimet.2003.11.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Following air sampling fungal DNA needs to be extracted and purified to a state suitable for laboratory use. Our laboratory has developed a simple method of extraction and purification of fungal DNA appropriate for enzymatic manipulation and Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective for use with multiple fungal organisms. Published by Elsevier B.V.

引用

页码：431 / 434

页数：4

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